Role of epidermal γδ T-cell-derived interleukin 13 in the skin-whitening effect of Ginsenoside F1
Role of epidermal γδ T-cell-derived interleukin 13 in the skin-whitening effect of Ginsenoside F1
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DOI：
10.1111/exd.12531
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发表时间：
2014-11-01
影响因子：
3.6
通讯作者：
Kim, Kwangmi
中科院分区：
文献类型：
--
作者：
Han, Jiyeon;Lee, Eunkyung;Kim, Kwangmi
人参皂苷F1（Ginsenoside F1，GF 1）是人参皂苷Rg 1的代谢产物。虽然GF 1对皮肤生理学有几个好处，但GF 1对皮肤色素沉着的影响尚未报道。我们发现，含有0.1%GF1的霜剂在应用8周后对人工晒黑的人皮肤显示出显著的美白效果。然而，GF 1不抑制酪氨酸酶或多巴色素互变异构酶（DCT）在正常人表皮黑素细胞（NHEMs）或共培养的NHEMs/正常人表皮角质形成细胞的mRNA表达。有趣的是，GF 1增强了人表皮T细胞产生白细胞介素13（IL-13）。IL-13显著降低NHEM中酪氨酸酶和DCT的mRNA表达和蛋白量，并降低黑色素合成活性，导致NHEM颗粒可见变亮。这些结果表明，由表皮T细胞的GF 1产生的IL-13的增强可能通过抑制酪氨酸酶和DCT在GF 1的皮肤美白效果中发挥作用。
Ginsenoside F1 (GF1) is a metabolite of ginsenoside Rg1. Although GF1 has several benefits for skin physiology, the effect of GF1 on skin pigmentation has not been reported. We found that a cream containing 0.1% GF1 showed a significant whitening effect on artificially tanned human skin after 8weeks of application. However, GF1 did not inhibit mRNA expression of tyrosinase or dopachrome tautomerase (DCT) in normal human epidermal melanocytes (NHEMs) or cocultured NHEMs/normal human epidermal keratinocytes. Interestingly, GF1 enhanced production of interleukin 13 (IL-13) from human epidermal T cells. IL-13 significantly reduced the mRNA expression and protein amount of both tyrosinase and DCT and reduced melanin synthesis activities in NHEMs, resulting in visible brightening of NHEM pellet. These results suggest that enhancement of IL-13 production by GF1 from epidermal T cells might play a role in the skin-whitening effect of GF1 via the suppression of tyrosinase and DCT.