Cc2d1b Contributes to the Regulation of Developmental Myelination in the Central Nervous System.
Cc2d1b Contributes to the Regulation of Developmental Myelination in the Central Nervous System.
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DOI：
10.3389/fnmol.2022.881571
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发表时间：
2022
影响因子：
4.8
通讯作者：
Poitelon Y
中科院分区：
文献类型：
--
作者：
Acheta J;Hong J;Jeanette H;Brar S;Yalamanchili A;Feltri ML;Manzini MC;Belin S;Poitelon Y
许多研究表明，髓鞘形成是细胞外信号和复杂的转录因子网络之间相互作用的结果。然而，识别和表征的全部剧目的转录因子，调节髓鞘仍然是不完整的。CC 2D 1B是Lgd/CC 2D 1蛋白家族的成员，在中枢和外周神经系统的髓鞘形成细胞中高度表达。此外，CC 2D 1B的缺乏限制了体外髓鞘形成。在这里，我们建议描绘的功能，CC 2D 1B在髓鞘细胞在体内的中枢和外周神经系统的髓鞘形成过程中。我们使用Cc 2d 1b组成型基因敲除小鼠模型，然后进行坐骨神经半薄切片和视神经的电子显微镜照片的形态学分析。我们还进行了冠状脑切片的免疫组织化学研究。所有分析均在30日龄时进行。在外周神经系统中，与对照动物相比，Cc 2d 1b消融动物未显示任何髓鞘厚度差异。在中枢神经系统中，免疫组织学研究未显示皮质、胼胝体和纹状体中少突胶质细胞数量或髓鞘蛋白水平的任何差异。然而，与大直径有髓纤维的对照动物（0.832 ± 0.016）相比，视神经显示出髓鞘形成不足（0.844 ± 0.022）。我们发现CC 2D 1B在中枢神经系统的发育髓鞘形成中起作用。这些结果表明，CC 2D 1B可能有助于在视神经少突胶质细胞髓鞘形成过程中的基因调控。
Numerous studies have indicated that myelination is the result of the interplay between extracellular signals and an intricate network of transcription factors. Yet, the identification and characterization of the full repertoire of transcription factors that modulate myelination are still incomplete. CC2D1B is a member of the Lgd/CC2D1 family of proteins highly expressed in myelinating cells in the central and peripheral nervous systems. In addition, the absence of CC2D1B limits myelin formation in vitro. Here we propose to delineate the function of CC2D1B in myelinating cells during developmental myelination in vivo in the central and peripheral nervous systems. We used a Cc2d1b constitutive knockout mouse model and then performed morphological analyses on semithin sections of sciatic nerves and electron micrographs of optic nerves. We also performed immunohistological studies on coronal brain sections. All analyses were performed at 30 days of age. In the peripheral nervous system, animals ablated for Cc2d1b did not show any myelin thickness difference compared to control animals. In the central nervous system, immunohistological studies did not show any difference in the number of oligodendrocytes or the level of myelin proteins in the cortex, corpus callosum, and striatum. However, optic nerves showed a hypomyelination (0.844 ± 0.022) compared to control animals (0.832 ± 0.016) of large diameter myelinated fibers. We found that CC2D1B plays a role in developmental myelination in the central nervous system. These results suggest that CC2D1B could contribute to gene regulation during oligodendrocytes myelination in optic nerves.
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DOI：
10.18632/aging.101032
发表时间：
2016-09-12
期刊：
Aging
影响因子：
--
作者：
Choi CI;Yoo KH;Hussaini SM;Jeon BT;Welby J;Gan H;Scarisbrick IA;Zhang Z;Baker DJ;van Deursen JM;Rodriguez M;Jang MH
通讯作者：
Jang MH
影响因子：
9.2
作者：
Childress, Jennifer L.;Acar, Melih;Haider, Georg
通讯作者：
Haider, Georg
影响因子：
11.8
作者：
Gallagher, Ciara M.;Knoblich, Juergen A.
通讯作者：
Knoblich, Juergen A.
影响因子：
5.3
作者：
Nakamura, Akito;Naito, Mikihiko;Fujita, Naoya
通讯作者：
Fujita, Naoya
影响因子：
4.7
作者：
Fan LW;Bhatt A;Tien LT;Zheng B;Simpson KL;Lin RC;Cai Z;Kumar P;Pang Y
通讯作者：
Pang Y