The effects of the Bowman-Birk protease inhibitor on c-myc expression and cell proliferation in the unirradiated and irradiated mouse colon.
The effects of the Bowman-Birk protease inhibitor on c-myc expression and cell proliferation in the unirradiated and irradiated mouse colon.
复制标题
Bowman-Birk 蛋白酶抑制剂对未辐射和辐射小鼠结肠中 c-myc 表达和细胞增殖的影响。
DOI：
10.1016/0304-3835(90)90257-x
复制
发表时间：
1990
期刊：
影响因子：
9.7
通讯作者：
Kennedy,AR
中科院分区：
文献类型：
--
作者：
StClair,WH;Billings,PC;Kennedy,AR
在口服和不口服 Bowman-Birk 蛋白酶抑制剂 (BBI) 的情况下，在 X 射线照射后，监测小鼠结肠粘膜中 c-myc RNA 和隐窝细胞增殖的水平。将小鼠分为4组并进行如下处理：（A）每日用水灌胃； (B) 每日用 BBI 灌胃； (C) 每日灌胃用水，并在第一次灌胃后1天进行12Gy的腹部照射； (D) 每天用 BBI 进行灌胃，并在第一次灌胃后 1 天进行 12 Gy 的腹部照射。在X射线照射后的不同时间收集样品，并采集升结肠样品用于隐窝细胞增殖分析。从剩余样品中除去粘膜并分离总细胞RNA。 X 射线照射后，c-myc mRNA 的水平经历了时间依赖性变化。 c-myc基因的表达在照射后1天和3周没有变化，但在X射线照射后1周显着升高。研究发现 BBI 可以抑制辐射引起的 c-myc mRNA 升高，同时对隐窝细胞增殖率或小鼠体重没有影响。
The levels of c-myc RNA and crypt cell proliferation were monitored in the mouse colonic mucosa following X-irradiation with and without oral administration of the Bowman-Birk protease inhibitor (BBI). Mice were divided into 4 groups and treated as follows: (A) daily gavage with water; (B) daily gavage with BBI; (C) daily gavage with water and 12 Gy of abdominal irradiation 1 day after the first gavage; (D) daily gavage with BBI and 12 Gy of abdominal irradiation 1 day after the first gavage. Samples were collected at various times after X-irradiation and ascending colon samples were taken for crypt cell proliferation analysis. The mucosa was removed from the remaining sample and total cellular RNA was isolated. The levels of c-myc mRNA underwent a time-dependent change following X-irradiation. Expression of the c-myc gene was unchanged at 1 day and 3 weeks after irradiation, but was markedly elevated at 1 week after X-irradiation. BBI was found to suppress the radiation induced elevation of c-myc mRNA, while having no effect on the rate of crypt cell proliferation or body weight of the mice.