DETERMINATION OF THE ORDER OF MUTATIONAL SITES GOVERNING L-ARABINOSE UTILIZATION IN ESCHERICHIA-COLI B-R BY TRANSDUCTION WITH PHAGE PLBT
DETERMINATION OF THE ORDER OF MUTATIONAL SITES GOVERNING L-ARABINOSE UTILIZATION IN ESCHERICHIA-COLI B-R BY TRANSDUCTION WITH PHAGE PLBT
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DOI：
10.1016/0042-6822(59)90125-4
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发表时间：
1959-01-01
期刊：
影响因子：
3.7
通讯作者：
ENGLESBERG, E
中科院分区：
文献类型：
--
作者：
GROSS, J;ENGLESBERG, E
用噬菌体Plbt进行3因子和4因子转导实验，确定了17株大肠杆菌B/r L-阿拉伯糖突变体的序列。在两对阿拉伯糖突变体之间的重组子中，观察到位于阿拉伯糖簇两侧的标记苏氨酸和亮氨酸的分类。阿拉伯糖突变体的位点排列成线性序列。阿拉伯糖突变体分为3个功能基团。A组(4个突变体)积累微量酮糖，并受L-阿拉伯糖抑制；B组(8个突变体)积累大量酮糖核酮糖，受L-阿拉伯糖抑制；C组(5个突变体)积累少量酮糖(多于A组)，并对L-阿拉伯糖产生抗性。通过流产转导试验将C组突变体与A、B组突变体区分开来。突变位点的顺序与按功能标准分组的比较表明，突变的顺序与它们所属的基团之间完全一致。涉及阿拉伯糖突变体的转导实验的特征是存在显著的负干扰。
The order of 17 L-arabinose mutants of Escherichia coli B/r has been determined by 3-and 4-factor transduction experiments with phage Plbt. The assortment of the markers threonine and leucine, located on either side of the arabinose cluster, was observed among recombinants between pairs of arabinose mutants. The sites of the arabinose mutants are arranged in a linear sequence. The arabinose mutants are divided into 3 functional groups. Group A (4 mutants) accumulates trace amounts of a keto sugar and is inhibited by L-arabinose; Group B (8 mutants) accumulates large quantities of the keto sugar, ribulose, and is inhibited by L-arabinose; Group C (5 mutants) accumulates small amounts of a keto sugar (more than Group A) and is resistant to L-arabinose. Group C mutants are distinguished from mutants in Groups A and B by abortive transduction tests. Comparison of the order of the mutant sites with the grouping by functional criteria demonstrates complete correspondence between the order of the mutants and the groups to which they belong. The transduction experiments involving pairs of arabinose mutants were characterized by the presence of marked negative interference.