Estimating age of humans based on telomere shortening
Estimating age of humans based on telomere shortening
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DOI：
10.1016/s0379-0738(02)00086-5
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发表时间：
2002-05-23
影响因子：
2.2
通讯作者：
Ikeda, N
中科院分区：
文献类型：
--
作者：
Tsuji, A;Ishiko, A;Ikeda, N
为了使用基于端粒缩短的DNA来估计年龄，我们使用外周血和血迹的Southern印迹分析来确定末端限制性片段（TRF）长度，作为端粒长度。所有血迹均在室温下储存5个月。TRF的平均长度明显地显示出随着年龄的增长而缩短的趋势。年龄估计公式基于平均TRF长度和受试者年龄之间的相关性。根据TRF长度计算的估计年龄在很大程度上取决于环境和遗传因素。然而，只要DNA保存良好，无论受试者的年龄如何，使用我们的方法都是可行的，并且可以粗略估计法医样本中不携带形态信息的受试者的年龄。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
To estimate age using DNA based on telomere shortening, we determined the terminal restriction fragment (TRF) length, as telomere length, using Southern blot analysis of peripheral human blood and blood stains. All blood stains had been stored at room temperature for 5 months. The average TRF length clearly showed a tendency to shortening with aging. The formula for age estimation was based on a correlation between average TRF length and age of the subjects. The estimated age calculated from TRF length widely depends on environmental and genetic factors. However, as long as the DNA is well preserved, use of our method is feasible regardless of age of the subject and can give a rough estimation of age of subjects in forensic samples that carry no morphological information. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.