Effect of needle geometry on flow rate and cell damage in the dispensing-based biofabrication process
Effect of needle geometry on flow rate and cell damage in the dispensing-based biofabrication process
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DOI：
10.1002/btpr.679
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发表时间：
2011-11-01
影响因子：
2.9
通讯作者：
Chen, Xiongbiao
中科院分区：
文献类型：
--
作者：
Li, Minggan;Tian, Xiaoyu;Chen, Xiongbiao
生物分配技术已广泛应用于生物制造过程中，以输送细胞悬浮液和生物材料来创建细胞接种构建体。在相同的操作条件下，锥形和圆柱形两种类型的分配针可以导致不同的材料流速和不同的细胞损伤百分比由机械力引起。在这项工作中，在生物分配系统中使用锥形和圆柱形针，分别开发的流量和细胞损伤百分比的数学模型，并进行了实验，以验证所开发的模型的有效性。模拟和实验都表明，锥形针产生更高的流量比圆柱形针在相同的压力条件下。因此，在锥形针中使用较低的压力可以实现与圆柱形针相同的流速。在相同的流速下，锥形针头中的细胞损伤低于圆柱形针头中的细胞损伤。采用组织工程中广泛应用的雪旺细胞和3T3成纤维细胞对细胞损伤模型进行了验证。所开发的模型的应用程序，以指定的工艺参数，包括针的几何形状和空气压力，对流速和细胞损伤百分比的影响，代表了一个显着的进步，生物纤维素工艺。该模型可用于优化工艺参数，以保持细胞活力并在基于分配的生物制品中实现所需的细胞分布。(C)2011年美国化学工程师学会生物技术。程序，，2011
Biodispensing techniques have been widely applied in biofabrication processes to deliver cell suspensions and biomaterials to create cell-seeded constructs. Under identical operating conditions, two types of dispensing needlestapered and cylindricalcan result in different flow rates of material and different cell damage percent induced by the mechanical forces. In this work, mathematical models of both flow rate and cell damage percent in biodispensing systems using tapered and cylindrical needles, respectively, were developed, and experiments were carried out to verify the effectiveness of the developed models. Both simulations and experiments show tapered needles produce much higher flow rates under the same pressure conditions than cylindrical needles. Use of a lower pressure in a tapered needle can therefore achieve the same flow rate as that in a cylindrical needle. At equivalent flow rates, cell damage in a tapered needle is lower than that in a cylindrical one. Both Schwann cells and 3T3 fibroblasts, which have been widely used in tissue engineering, were used to validate the cell damage models. Application of the developed models to specify the influence of process parameters, including needle geometry and air pressure, on the flow rate and cell damage percent represents a significant advance for biofabrication processes. The models can be used to optimize process parameters to preserve cell viability and achieve the desired cell distribution in dispensing-based biofabrication. (C) 2011 American Institute of Chemical Engineers Biotechnol. Prog.,, 2011