Otic placode cell specification and proliferation re regulated by Notch signaling in avian development
Otic placode cell specification and proliferation re regulated by Notch signaling in avian development
复制标题
鸟类发育中Notch信号重新调节耳基板细胞规范和增殖
DOI：
10.1002/dvdy.24291
复制
发表时间：
2015
影响因子：
2.5
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Shida;H.;Mende;M.;Takano-Yamamoto;T.;Osumi;N.;Streit;A.;Wakamatsu;Y.
背景：包括耳蜗-前庭神经节在内的整个内耳都是由一个简单的上皮细胞——耳基细胞形成的。基板的前体起源于位于未来后脑附近的外胚层的祖细胞池，在那里它们与未来的鳃外细胞和表皮细胞混合在一起。虽然分泌蛋白（如FGFs和wnt）在赋予个体身份方面的重要性已经得到了很好的研究，但这些不同命运的前体如何在局部分离却不太清楚。结果(1)Notch配体delta1和Notch靶标5‐2在发病前的部分区域表达，表明Notch信号通路活跃，这一点通过Notch报告基因得到了证实。(2)功能损失和功能增益的研究表明，Notch信号可以调节前祖细胞的增殖和特异性。结论Notch信号通路在禽类胚胎发育前决定细胞命运中具有新的功能。科学通报，2014(4):349 - 351。©2015 Wiley期刊公司
BackgroundThe entire inner ear including the cochlear‐vestibular ganglion arises from a simple epithelium, the otic placode. Precursors for the placode originate from a pool of progenitors located in ectoderm next to the future hindbrain, the pre‐otic field, where they are intermingled with future epibranchial and epidermal cells. While the importance of secreted proteins, such as FGFs and Wnts, in imparting otic identity has been well studied, how precursors for these different fates segregate locally is less well understood.Results(1) The Notch ligandDelta1and the Notch targetHes5‐2are expressed in a part of pre‐otic field before otic commitment, indicative of active Notch signaling, and this is confirmed using a Notch reporter. (2) Loss and gain‐of‐function approaches reveal that Notch signaling regulates both proliferation and specification of pre‐otic progenitors.ConclusionsOur results identify a novel function of Notch signaling in cell fate determination in the pre‐otic field of avian embryos.Developmental Dynamics 244:839–851, 2015. © 2015 Wiley Periodicals, Inc.