FEULGEN HYDROLYSIS - EFFECT OF ACID AND TEMPERATURE
FEULGEN HYDROLYSIS - EFFECT OF ACID AND TEMPERATURE
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DOI：
10.1177/14.8.601
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发表时间：
1966-01-01
影响因子：
3.2
通讯作者：
AIELLO, N
中科院分区：
文献类型：
--
作者：
DECOSSE, JJ;AIELLO, N
通过连续显微分光光度法测量正常人淋巴细胞的相对DNA含量，对两种福尔根水解方法（室温下5.0 [image] HCl和60[degree] C下1.0 [image] HCl）进行了检测。两种方法在最佳水解时的DNA含量相等。室温下在5.0 [image] HCl中水解，得到120 min的稳定峰值间隔。如初始实验中为每种方法所定义的，连续水解至最佳时间，表明在室温下在5.0 [image] HCl中水解提供了更可靠的技术。结果还表明，脱嘌呤主要依赖于酸而不是热，而进一步降解主要依赖于热而不是酸。
Two methods of Feulgen hydrolysis (5.0 [image] HCl at room temperature and 1.0 [image] HCl at 60[degree] C) were examined by serial microspectrophotometric measurements of the relative DNA content of normal human lymphocytes subjected to each hydrolysis. The DNA contents at optimum hydrolysis were equal by both methods. Hydrolysis in 5.0 [image] HCl at room temperature resulted in an interval of 120 min of stable peak values. Successive hydrolyses to the optimum time, as defined for each method in the initial experiment, indicated that hydrolysis in 5.0 [image] HC1 at room temperature provided a more reliable technique. The results also suggested that depurination is dependent primarily on acid rather than heat, while further degradation is dependent primarily on heat rather than acid.