Carcinoembryonic antigens: alternative splicing accounts for the multiple mRNAs that code for novel members of the carcinoembryonic antigen family.
Carcinoembryonic antigens: alternative splicing accounts for the multiple mRNAs that code for novel members of the carcinoembryonic antigen family.
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DOI：
10.1083/jcb.108.2.267
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发表时间：
1989-02
期刊：
影响因子：
--
通讯作者：
Kamarck ME
中科院分区：
文献类型：
--
作者：
Barnett TR;Kretschmer A;Austen DA;Goebel SJ;Hart JT;Elting JJ;Kamarck ME
最近克隆了编码癌胚抗原（CEA）和非特异性交叉反应抗原的完整cDNA，揭示了免疫球蛋白基因超家族中存在一个新的基因家族。我们已经报道了部分CEA cDNA和表达与针对天然CEA的商业抗体交叉反应的人抗原的L-细胞转染细胞系的分离（Kamarck，M.，J. Elting，J.哈特，S. Goebel，P. M. M. Rae，J. Nedwin，and T.巴内特。1987. Proc. Natl. Acad. Sci. USA. 84：5350-5354）。在这项研究中，我们描述了3.9-，3.7-，2.2-和1.8-kb的cDNA和23-kb的基因组转录单位，编码CEA基因家族的新成员的识别和克隆。这些克隆的DNA的DNA序列分析建立了一组四个选择性剪接的mRNA的存在，这是在几个肿瘤细胞系，在人胎肝，和L-细胞转染表达。推导出的编码的同种抗原的氨基酸序列显示出广泛的相似性，CEA和非特异性交叉反应抗原，但另外证明跨膜和胞质结构域。我们将该抗原家族的成员命名为跨膜CEA。跨膜CEA同种抗原与免疫球蛋白基因超家族的成员共享一般结构特征，并且可以特异性地与细胞粘附分子N-CAM（神经细胞粘附分子）和MAG（髓鞘相关糖蛋白）进行比较。
The recent cloning of complete cDNAs encoding carcinoembryonic antigen (CEA) and non-specific cross-reacting antigen has revealed the existence of a new gene family belonging to the immunoglobulin gene superfamily. We have reported the isolation of a partial CEA cDNA and of L-cell transfectant cell lines that express human antigens cross- reactive with commercial antibodies directed to native CEA (Kamarck, M., J. Elting, J. Hart, S. Goebel, P. M. M. Rae, J. Nedwin, and T. Barnett. 1987. Proc. Natl. Acad. Sci. USA. 84:5350-5354). In this study, we describe the identification and cloning of 3.9-, 3.7-, 2.2-, and 1.8-kb cDNAs and a 23-kb genomic transcription unit, which code for new members of the CEA gene family. DNA sequence analysis of these cloned DNAs establishes the existence of a set of four alternatively spliced mRNAs which are expressed in several tumor cell lines, in human fetal liver, and in L-cell transfectants. Deduced amino acid sequences of the encoded isoantigens show extensive similarity to CEA and nonspecific cross-reacting antigens, but in addition demonstrate transmembrane and cytoplasmic domains. We designate members of this antigen family transmembrane CEAs. The transmembrane CEA isoantigens share general structural characteristics with members of the immunoglobulin gene superfamily and can be specifically compared to the cell adhesion molecules, N-CAM (neural cell adhesion molecule) and MAG (myelin-associated glycoprotein).