PHENOLIC ANTIOXIDANTS OF DRIED SOYBEANS
PHENOLIC ANTIOXIDANTS OF DRIED SOYBEANS
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DOI：
10.1111/j.1365-2621.1978.tb02353.x
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发表时间：
1978-01-01
影响因子：
3.9
通讯作者：
PRATT, DE
中科院分区：
文献类型：
--
作者：
HAMMERSCHMIDT, PA;PRATT, DE
研究了全干大豆种子以确定其抗氧化活性的性质。通过亚油酸和β-胡萝卜素的偶联氧化中胡萝卜素漂白的速率来测量活性。胡萝卜素大豆甲醇提取物具有较强的抗氧化能力。纸层析和葡聚糖凝胶柱层析得到不完全的决议的个别成分。在分离该组分时使用整个甲醇提取物的TLC，而不是单独的柱级分。三条荧光带具有主要活性。进一步分析了最有效的复合物。用薄层层析法将配合物分离为4个荧光组分。一种化合物被确定为负责由母带所表现出的抗氧化活性。进一步通过纸层析、显色喷雾试验和紫外光谱分析，证明该抗氧化剂为苦杏仁戊糖苷。
Whole dried soybean seeds were investigated to determine the nature of their antioxidant activity. Activity was measured by rates of carotene bleaching in the coupled oxidation of linoleic acid and .beta.-carotene. Methanolic extracts of the soybeans possessed potent antioxidant ability. Paper and Sephadex column chromatography gave incomplete resolution of individual components. TLC of the whole methanolic extract rather than individual column fractions was used in isolating this component. Three fluorescent bands possessed the major activity. The most potent complex was further analyzed. TLC was used to separate the complex into 4 fluorescent components. One compound was determined to be responsible for the antioxidant activity exhibited by the parent band. Further analysis of this component by paper chromatography, chromogenic spray reagent tests and UV spectral analyses indicated that the antioxidant was an isoflavone pentoside.