Novel electrical stimulation sets the cultured myoblast contractile function to 'on'
Novel electrical stimulation sets the cultured myoblast contractile function to 'on'
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DOI：
10.1159/000099123
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发表时间：
2006-01-01
期刊：
影响因子：
5
通讯作者：
Yuge, Louis
中科院分区：
文献类型：
--
作者：
Kawahara, Yumi;Yamaoka, Kaoru;Yuge, Louis
目的：在本研究中，电刺激的效果进行了检查的能力，诱导成肌细胞分化过程中的形态，生理和分子生物学效应。方法：采用新开发的电刺激方法，在培养第6、8、10和12天对L 6大鼠成肌细胞进行电刺激。结果：电刺激加速了肌管的出现，随后产生自发收缩的肌纤维。膜电位测定表明，收缩细胞具有功能性离子通道和细胞间缝隙连接通讯。在电刺激的细胞中，还观察到MyoD家族和M-cadherin的表达增强。连接蛋白43的表达增加，并保持在高水平的电刺激的细胞。结论：这是首次证明在体外诱导成肌细胞自发收缩肌纤维的间歇刺激。这种诱导成肌细胞分化的新方法代表了细胞治疗的重要进展。版权所有（c）2006 S. Karger AG，巴塞尔。
Objective: In the present study, the effect of electrical stimulation was examined for the ability to induce morphological, physiological, and molecular biological effects on myoblasts during cell differentiation. Methods: L6 rat myoblasts were electrically stimulated by newly developed methods on culture days 6, 8, 10 and 12. Results: This electrical stimulation accelerated the appearance of myotubes, and subsequently produced spontaneously contracting muscle fibers. Measurement of membrane potential showed that the contracting cell had functional ion channels and gap junctional intercellular communication. In the electrically stimulated cells, an enhanced expression of MyoD family and M-cadherin was also observed. Expression of connexin 43 was increased and maintained at a high level in the electrically stimulated cells. Conclusion: This is the first demonstration of in vitro induction of myoblasts in spontaneously contractile muscle fibers by intermittent stimulation. This novel method for induction of myoblast differentiation represents an important advance in cell therapy. Copyright (c) 2006 S. Karger AG, Basel.