dsRNA-mediated resistance to Beet Necrotic Yellow Vein Virus infections in sugar beet (Beta vulgaris L. ssp vulgaris)
dsRNA-mediated resistance to Beet Necrotic Yellow Vein Virus infections in sugar beet (Beta vulgaris L. ssp vulgaris)
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DOI：
10.1007/s11032-006-9030-5
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发表时间：
2006-11-01
影响因子：
3.1
通讯作者：
Gielen, Jan
中科院分区：
文献类型：
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作者：
Lennefors, Britt-Louise;Savenkov, Eugene I.;Gielen, Jan
甜菜根枯病是由甜菜坏死性黄脉病毒(BNYVV)引起的一种严重危害甜菜的病害。利用抗BNYVV的甜菜品种通常被认为是在根瘤病侵染的田地保持有利可图产量的唯一途径。作为天然抗性的替代，我们探索了转基因表达病毒dsRNA来工程抗性根瘤病。表达BNYVV复制酶基因0.4kb片段反向重复的转基因植株对不同遗传株的BNYVV表现出高水平的抗性。在温室和田间的高侵染压力和较长的生育期内保持了抗性。抗性植株在根中积累了极低数量的转基因mRNA和大量的相应siRNA，说明了RNA沉默是潜在的机制。转基因抗性比天然的根瘤病抗性来源要好得多，因此为培育抗病甜菜品种提供了一种有吸引力的选择。
Rhizomania, one of the most devastating diseases in sugar beet, is caused by Beet Necrotic Yellow Vein Virus (BNYVV) belonging to the genus Benyvirus. Use of sugar beet varieties with resistance to BNYVV is generally considered as the only way to maintain a profitable yield on rhizomania-infested fields. As an alternative to natural resistance, we explored the transgenic expression of viral dsRNA for engineering resistance to rhizomania. Transgenic plants expressing an inverted repeat of a 0.4 kb fragment derived from the BNYVV replicase gene displayed high levels of resistance against different genetic strains of BNYVV when inoculated using the natural vector, Polymyxa betae. The resistance was maintained under high infection pressures and over prolonged growing periods in the greenhouse as well as in the field. Resistant plants accumulated extremely low amounts of transgene mRNA and high amounts of the corresponding siRNA in the roots, illustrative of RNA silencing as the underlying mechanism. The transgenic resistance compared very favourably to natural sources of resistance to rhizomania and thus offers an attractive alternative for breeding resistant sugar beet varieties.