INDUCIBLE BINDING OF A FACTOR TO THE C-FOS REGULATORY REGION
INDUCIBLE BINDING OF A FACTOR TO THE C-FOS REGULATORY REGION
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DOI：
10.1073/pnas.84.5.1272
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发表时间：
1987-03-01
影响因子：
11.1
通讯作者：
COCHRAN, BH
中科院分区：
文献类型：
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作者：
HAYES, TE;KITCHEN, AM;COCHRAN, BH
c-fos基因在静止的BALB/c-3T3细胞中响应血清、血小板衍生生长因子或v-sis转化细胞的条件培养基而迅速而短暂地激活。这种激活发生在转录水平，在没有新蛋白质合成的情况下。通过凝胶电泳dna结合实验，我们发现在v-sis转化细胞的条件培养基处理20分钟内，BALB/c-3T3细胞具有dna结合活性。DNA甲基化干扰实验表明，该因子与人类c-fos基因转录起始位点上游约346个碱基对的序列结合。胰岛素、表皮生长因子和12-肉豆蔻酸13-乙酸佛波不能诱导这种dna结合因子。蛋白质合成抑制剂不能阻断这种活性的诱导。我们认为该因子在静止细胞中以非活性形式存在，其结合活性在适当的细胞外诱导剂的作用下被激活。
The c-fos gene is rapidly and transiently activated in quiescent BALB/c-3T3 cells in response to serum, platelet-derived growth factor or conditioned medium from v-sis-transformed cells. This activation occurs at the level of transcription and in the absence of new protein synthesis. Using a gel electrophoresis DNA-binding assay, we have found a DNA-binding activity in BALB/c-3T3 cells that is induced within 20 min of treatment with conditioned medium from v-sis-transformed cells. A DNA methylation interference assay has shown that this factor binds to a sequence approximately 346 base pairs upstream of the transcription initiation site of the human c-fos gene. Insulin, epidermal growth factor, and phorbol 12-myristate 13-acetate fail to induce this DNA-binding factor. Protein synthesis inhibitors do not block the induction of this activity. We propose that this factor preexists in an inactive form in quiescent cells and that its binding activity is activated in response to appropriate extracellular inducers.