Introduction of human immunodeficiency virus infection among parenteral drug abusers in Sardinia: a seroepidemiologic study.
Introduction of human immunodeficiency virus infection among parenteral drug abusers in Sardinia: a seroepidemiologic study.
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撒丁岛肠外药物滥用者中人类免疫缺陷病毒感染的介绍：一项血清流行病学研究。
DOI：
10.1093/oxfordjournals.aje.a114924
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发表时间：
1988
影响因子：
5
通讯作者：
Balestrieri,A
中科院分区：
文献类型：
--
作者：
Farci,P;Novick,DM;Lai,ME;Orgiana,G;Strazzera,A;Beatrice,ST;DesJarlais,DC;Balestrieri,A
材料和方法 1978 年 1 月至 1986 年 12 月期间，对 965 名连续的肠外药物滥用者进行了医学评估 (6) 并转诊至卡利亚里大学（意大利撒丁岛）内科进行美沙酮维持治疗。所有胃肠外滥用药物的患者都有资格接受评估。由于 1982 年开设了第二个治疗注射药物滥用的中心，因此 1982 年至 1986 年收治的患者数量有所减少。第二个治疗中心在大多数方面与我们的设施相似，但不隶属于大学。患者可以自由选择任一中心。每次访视时，从每个患者处获取血清并保存在-20℃下。对1978-1984年收集的血清进行回顾性测定抗HIV；从1985年开始，它是前瞻性确定的。使用商业试剂盒（雅培实验室，北芝加哥，伊利诺斯州）通过酶联免疫测定（ELISA）测定抗 HIV 抗体。该测定一式两份进行。仅当 ELISA 在两次不同的情况下出现反应时才被视为阳性。所有 ELISA 呈阳性的样本均被送往纽约市卫生部逆转录病毒学实验室进行蛋白质印迹检测。该方法是 Tsang 等人 (7) 的方法，如果条带在以下三个区域中的任意两个区域可见，则认为蛋白质印迹是有反应的：p24、gp41 和 gp120。具有反应性蛋白质印迹的患者被认为抗 HIV 血清呈阳性。 Student t 检验和卡方
MATERIALS AND METHODSBetween January 1978 and December 1986, 965 consecutive individual parenteral drug abusere had medical evaluations (6) and referrals for methadone maintenance treatment at the Department of Internal Medicine of the University of Cagliari (Sardinia, Italy). All patients abusing drugs parenterally were eligible for evaluation. Fewer patients were seen from 1982 to 1986 due to the opening in 1982 of a second center for treatment of parenteral drug abusere. The second treatment center was similar to our facility in most respects but was not affiliated with the University. Patients were free to select either center. At each visit, sera were obtained from each patient and stored at-20 C. Anti-HIV was determined retrospectively in sera collected from 1978-1984; starting in 1985 it was determined prospectively. Anti-HIV was determined by enzyme-Linked immunoassay (ELISA) using commercial kits (Abbott Laboratories, North Chicago, IL). The assay was done in duplicate. An ELISA was considered positive only if it was reactive on two separate occasions. All samples positive by ELISA were sent for Western blot testing under code to the Retrovirology Laboratory of the New York City Department of Health. The method was that of Tsang et al.(7), and the Western blot was considered reactive if bands were visible at any two of the following three regions: p24, gp41, and gpl20. Patients with reactive Western blots are considered seropositive for anti-HIV. The Student t test and the chi-square