Characterization of gingerol-related compounds in ginger rhizome (Zingiber officinale Rosc.) by high-performance liquid chromatography/electrospray ionization mass spectrometry
Characterization of gingerol-related compounds in ginger rhizome (Zingiber officinale Rosc.) by high-performance liquid chromatography/electrospray ionization mass spectrometry
复制标题
DOI：
10.1002/rcm.2140
复制
发表时间：
2005-01-01
影响因子：
2
通讯作者：
Gang, DR
中科院分区：
文献类型：
--
作者：
Jiang, HL;S贸lyom, AM;Gang, DR
本研究旨在确定液相色谱/电喷雾串联质谱（LC/ESI-MS/MS）结合二极管阵列检测器在鉴定生姜根茎粗提物中姜辣素相关化合物中的应用。利用（-）-和（+）ESI-MS/MS中化合物的裂解行为来推断和确认几组化合物的化学结构，包括姜辣素、甲基姜辣素、姜辣素乙酸酯、姜烯酚、姜辣醇、姜二醇、单乙酰姜二醇和二乙酰姜二醇以及脱氢姜二酮。使用不同波长的二极管阵列检测来确认基于MS/MS的鉴别。从生姜甲醇粗提物中共鉴定出31种姜酚类化合物。其中3个化合物为新化合物。这项研究表明，LC/ESI-MS/MS是一个强大的在线工具，用于鉴定姜辣素相关化合物，特别是热不稳定的化合物，不能很容易地检测到GC/MS分析。版权所有（C）2005约翰威利父子有限公司
This study sought to determine the utility of liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) coupled with diode array detection in identifying gingerol-related compounds from crude extracts of ginger rhizome. The fragmentation behaviors of compounds in both (-)- and (+)ESI-MS/MS were used to infer and confirm the chemical structures of several groups of compounds, including the gingerols, methylgingerols, gingerol acetates, shogaols, paradols, gingerdiols, mono- and diacetyl gingerdiols, and dehydrogingerdiones. Diode array detection at different wavelengths was used to confirm MS/MS-based identification. In total, 31 gingerol-related compounds were identified from the methanolic crude extracts of fresh ginger rhizome in this study. Three of these compounds were found to be new compounds. This study demonstrated that LC/ESI-MS/MS is a powerful on-line tool for identification of gingerol-related compounds, especially for thermally labile compounds that cannot be readily detected by GC/MS analysis. Copyright (C) 2005 John Wiley & Sons, Ltd.