A restriction enzyme-based assay to distinguish between avian hemosporidians
A restriction enzyme-based assay to distinguish between avian hemosporidians
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DOI：
10.1645/ge-3412rn
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发表时间：
2005-06-01
影响因子：
1.3
通讯作者：
Fleischer, RC
中科院分区：
文献类型：
--
作者：
Beadell, JS;Fleischer, RC
我们描述了一种可靠且相对便宜的方法，用于检测和区分常用研究的禽血寄生虫属 Haemoproteus、Plasmodium 和 Leitcocytozooll。该测定利用了通过对两个 Haemoproteus 和三个 Plosmodium 谱系的完整线粒体基因组进行测序而鉴定出的 Haemoproteus 特异性限制位点，以及在白细胞虫属 (Leucocytozoon spp) 中鉴定出的邻近属特异性限制位点。该测定对每个红细胞约 8 x 10(-6) 的模拟寄生虫敏感，并且在区分从受感染宿主的广泛地理和分类样本中分离的寄生虫属方面具有 100% 的准确度。
We describe a reliable and relatively inexpensive method for detecting and differentiating between the commonly studied avian blood parasite genera Haemoproteus, Plasmodium, and Leitcocytozooll. The assay takes advantage of a Haemoproteus-specific restriction site identified by sequencing full rnitochondrial genomes from two Haemoproteus and three Plosmodium lineages and an adjacent, genus-specific restriction site identified in Leucocytozoon spp. The assay was sensitive to simulated parasitemas of approximately 8 x 10(-6) per erythrocyte and was 100% accurate in differentiating between parasite genera isolated from a broad geographical and taxonomic sampling of infected hosts.