Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method
Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated isothermal amplification method
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DOI：
10.1016/j.jcv.2007.02.001
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发表时间：
2007-05-01
影响因子：
8.8
通讯作者：
Yoshikawa, Tetsushi
中科院分区：
文献类型：
--
作者：
Ihira, Masaru;Akimoto, Shiho;Yoshikawa, Tetsushi
背景：人类疱疹病毒6 （HHV-6）感染监测需要一种更快速、更简便的方法。环介导等温扩增法（LAMP）在等温条件下检测病毒DNA具有高特异性、高效率和高速度。LAMP只需要医院实验室提供的简单设备。目的：我们评估LAMP作为直接从患者血清中检测HHV-6 DNA的方法。结果：无热变性的HHV-6 LAMP方案灵敏度为1000拷贝/管；热变性检测10拷贝/管。对300份发热儿童血清样本进行了分析。以HHV-6分离作为HHV-6感染的定义，不提取DNA的HHV-6 LAMP法的敏感性为95.5%，特异性为95.2%，阳性预测值为94.0%，阴性预测值为96.4%。结论：改良的HHV-6 LAMP直接检测血清中HHV-6 DNA可用于宫颈炎（ES）的快速诊断。(c) 2007 Elsevier B.V.版权所有
Background: A more rapid and easier method is needed for monitoring human herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification method (LAMP) can detect viral DNA with high specificity, efficiency, and speed under isothermal conditions. LAMP requires only simple equipment that is available in hospital laboratories.Objectives: We evaluated LAMP as a means of detecting HHV-6 DNA directly from patients' sera.Results: The sensitivity of the HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat denaturation 10 copies/tube were detected. Three hundred serum samples from children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 infection, the sensitivity, specificity, positive predictive value, and negative predictive value of the HHV-6 LAMP method without DNA extraction were 95.5%, 95.2%, 94.0%, and 96.4%, respectively.Conclusion: Direct detection of HHV-6 DNA in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem subitum (ES). (c) 2007 Elsevier B.V. All rights reserved.