Detection and Isolation of Airborne Influenza A H3N2 Virus Using a Sioutas Personal Cascade Impactor Sampler.
Detection and Isolation of Airborne Influenza A H3N2 Virus Using a Sioutas Personal Cascade Impactor Sampler.
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DOI：
10.1155/2013/656825
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发表时间：
2013-01-01
期刊：
影响因子：
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通讯作者：
Loeb, Julia C
中科院分区：
文献类型：
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作者：
Lednicky, John A;Loeb, Julia C
我们呼吸的空气中含有可引起传染性呼吸道疾病的微生物。2013年2月，一间公寓的两名住户被诊断出患有流感，之后，研究人员利用两种不同类型的主动空气采样器（Sioutas个人级联冲击采样器（PCIS）和SKC生物采样器）来检测和分离空气中的流感病毒。PCIS通过冲击聚四氟乙烯过滤器收集大小分级颗粒，而SKC生物采样器在液体介质中收集空气中的颗粒。两种空气采样器均采集到H3N2流感病毒。PCIS在一名病人附近收集了一系列含有流感病毒的颗粒大小，但当采样器放置在较远的地方时，只收集了超细颗粒。在SKC生物采样器的液体收集介质和一些PCIS过滤器中存在活病毒。这些发现表明，流感患者会产生含有活病毒的超细气溶胶颗粒。
The air we breathe contains microorganisms that can cause infectious respiratory diseases. After two occupants of an apartment were diagnosed with influenza in February of 2013, efforts were made to detect and isolate airborne influenza virus using two different types of active air samplers: a Sioutas Personal Cascade Impactor Sampler (PCIS) and an SKC BioSampler. The PCIS collects size-fractionated particles by impaction on polytetrafluoroethylene filters, whereas the SKC BioSampler collects airborne particles in liquid media. Influenza H3N2 virus was collected by both types of air samplers. The PCIS collected a range of particle sizes containing influenza virus near one of the sick individuals but only ultrafine particles when the samplers were positioned farther away. Viable virus was present in the liquid collection media of the SKC BioSampler and some PCIS filters. These findings suggest that influenza patients produce ultrafine aerosol particles that contain viable virus.