Actin Polymerization Mediated by AtFH5 Directs the Polarity Establishment and Vesicle Trafficking for Pollen Germination in Arabidopsis
Actin Polymerization Mediated by AtFH5 Directs the Polarity Establishment and Vesicle Trafficking for Pollen Germination in Arabidopsis
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AtFH5 介导的肌动蛋白聚合指导拟南芥花粉萌发的极性建立和囊泡运输
DOI：
10.1016/j.molp.2018.09.004
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发表时间：
2018-11-05
期刊：
影响因子：
27.5
通讯作者：
Ren, Haiyun
中科院分区：
文献类型：
--
作者：
Liu, Chang;Zhang, Yi;Ren, Haiyun
花粉萌发过程对开花植物的繁殖至关重要，但花粉粒形成极性和选择萌发地点的机制尚不清楚。在这项研究中，我们报道了一种formin家族蛋白AtFH5定位于囊泡，并在花粉萌发过程中在lifeact - megfp标记的肌动蛋白丝之前旋转。AtFH5在质膜上的易位在种子萌发前在预期萌发位点启动了项圈样肌动蛋白结构的组装。遗传和药理学证据进一步揭示了AtFH5标记的囊泡的移动性与肌动蛋白丝的聚合之间的相互依存关系：囊泡定位的AtFH5促进肌动蛋白的组装，而肌动蛋白丝的聚合和延伸反过来又对花粉粒中AtFH5标记的囊泡的移动性至关重要。总之，我们的工作揭示了花粉萌发过程中极性建立和囊泡迁移的分子机制。
The process of pollen germination is crucial for flowering plant reproduction, but the mechanisms through which pollen grains establish polarity and select germination sites are not well understood. In this study, we report that a formin family protein, AtFH5, is localized to the vesicles and rotates ahead of Lifeact-mEGFP-labeled actin filaments during pollen germination. The translocation of AtFH5 to the plasma membrane initiates the assembly of a collar-like actin structure at the prospective germination site prior to germination. Genetic and pharmacological evidence further revealed an interdependent relationship between the mobility of AtFH5-labeled vesicles and the polymerization of actin filaments: vesicle-localized AtFH5 promotes actin assembly, and the polymerization and elongation of actin filaments, in turn, is essential for the mobility of AtFH5-labeled vesicles in pollen grains. Taken together, our work revealed a molecular mechanism underlying the polarity establishment and vesicle mobility during pollen germination.