Surface plasmon resonance/surface plasmon enhanced fluorescence: An optical technique for the detection of multicomponent macromolecular adsorption at the solid/liquid interface
Surface plasmon resonance/surface plasmon enhanced fluorescence: An optical technique for the detection of multicomponent macromolecular adsorption at the solid/liquid interface
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DOI：
10.1021/la025578w
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发表时间：
2002-08-06
期刊：
影响因子：
3.9
通讯作者：
Gast, AP
中科院分区：
文献类型：
--
作者：
Roy, S;Kim, JH;Gast, AP
我们描述了一种光学技术，用于测量存在多种物质时固/液界面上的大分子吸附。该技术将表面等离子体共振 (SPR) 与同步表面等离子体增强荧光 (SPEF) 相结合。相对容易的构建以及 SPR 和 SPEF 信号之间的线性相关性使得该技术适合共吸附研究或多配体结合实验。在这里，我们通过生物素/抗生物素蛋白/BSA“三明治”实验展示了该技术的实用性。然后，我们应用 SPR/SPEF 同时监测与底物表面相互作用的蛋白酶的酶吸附和底物裂解。
We describe an optical technique for the measurement of macromolecular adsorption at the solid/liquid interface when multiple species are present. The technique combines surface plasmon resonance (SPR) with simultaneous surface plasmon enhanced fluorescence (SPEF). The relative ease of construction and linear correlation between SPR and SPEF signals make the technique amenable for coadsorption studies or multiple ligand binding experiments. Here, we demonstrate the utility of the technique with a biotin/avidin/BSA "sandwich" experiment. We then apply SPR/SPEF for the simultaneous monitoring of enzyme adsorption and substrate cleavage of a protease interacting with a substrate surface.