Agromyces flavus sp nov., an actinomycete isolated from soil
Agromyces flavus sp nov., an actinomycete isolated from soil
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DOI：
10.1099/ijs.0.023242-0
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发表时间：
2011-07-01
影响因子：
2.8
通讯作者：
Yu, Li-Yan
中科院分区：
文献类型：
--
作者：
Chen, Jie;Chen, Hua-Mei;Yu, Li-Yan
从青藏高原采集的土壤样品中分离到一株革兰氏阳性、非运动型菌株，命名为CPCC 202695（T）。菌株CPCC 202695 T在细胞壁中含有鼠李糖、葡萄糖和半乳糖作为诊断糖，在肽聚糖中含有2，4-二氨基丁酸、丙氨酸、谷氨酸和甘氨酸。极性脂质由二磷脂酰甘油、磷脂酰甘油和两种未知糖脂组成。主要脂肪酸为anteiso-C（15：0）（34.2%）、iso-C（15：0）（19.8%）、iso-C（16：0）（12.7%）和anteiso-C（17：0）（11.1%）。16 S rRNA基因序列与已知的Agromyces属模式菌株的同源性为94.2-97.1%，表明菌株CPCC 202695（T）属于Agromyces属。DNA DNA相关性清楚地将菌株CPCC 202695（T）与其最近的亲属分开。表型和基因型数据表明菌株CPCC 202695（T）代表Agromyces属的一个新种，并建议将其命名为Agromyces flavus sp. nov.。模式菌株为CPCC 202695（T）（=KCTC 19578（T）=CCM 7623（T））。
A Gram-positive, non-motile strain, designated CPCC 202695(T), was isolated from a soil sample collected from the Qinghai-Tibet plateau, north-west China. Strain CPCC 202695T contained rhamnose, glucose and galactose in the cell wall as diagnostic sugars and 2,4-diaminobutyric acid, alanine, glutamic acid and glycine in the peptidoglycan. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. MK-12 was the predominant menaquinone and anteiso-C(15:0) (34.2%), iso-C(15:0) (19.8%), iso-C(16:0) (12.7%) and anteiso-C(17:0) (11.1%) were the major fatty acids. 16S rRNA gene sequence similarities (94.2-97.1%) between the isolate and the type strains of recognized species of the genus Agromyces indicated that strain CPCC 202695(T) was a member of the genus Agromyces. DNA DNA relatedness clearly separated strain CPCC 202695(T) from its closest relatives. The phenotypic and genotypic data demonstrated that strain CPCC 202695(T) represents a novel species of the genus Agromyces, for which the name Agromyces flavus sp. nov. is proposed. The type strain is CPCC 202695(T) (=KCTC 19578(T) =CCM 7623(T)).