Expression and evaluation of Chikungunya virus E1 and E2 envelope proteins for serodiagnosis of Chikungunya virus infection.
Expression and evaluation of Chikungunya virus E1 and E2 envelope proteins for serodiagnosis of Chikungunya virus infection.
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DOI：
10.3349/ymj.2008.49.5.828
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发表时间：
2008-10-31
影响因子：
2.4
通讯作者：
Park S
中科院分区：
文献类型：
--
作者：
Cho B;Jeon BY;Kim J;Noh J;Kim J;Park M;Park S
基孔肯雅病毒（CHIKV）引起CHIKV热的地方性或流行性爆发，CHIKV热是非洲、印度、东南亚以及最近南欧的蚊子传播的病毒性疾病。目前，血清学诊断试验，如血凝抑制试验（HI试验），内部IgM捕获酶联免疫吸附试验（ELISA）和间接免疫荧光试验用于基孔肯雅热的诊断，这些试验基于全病毒抗原。在杆状病毒表达系统中表达用于基孔肯雅热的CHIKV特异性血清学诊断试剂的CHIKV E1和E2包膜蛋白。通过间接IgM捕获ELISA，使用来自Marcel Merieux的患者血清组评估重组CHIKV E1和E2包膜蛋白的血清反应性。重组CHIKV E1和E2包膜蛋白的敏感性分别为77.5%和90%。CHIKV E1和E2包膜蛋白的特异性均为100%。重组CHIKV E1和E2包膜蛋白可作为CHIKV感染的诊断试剂。
Chikungunya virus (CHIKV) causes endemic or epidemic outbreaks of CHIKV fever, which is a mosquitoe-transmitted viral disease in Africa, India, South-East Asia, and recently Southern Europe. Currently, serological diagnostic tests such as hemagglutination inhibition test (HI test), in-house IgM capture enzyme-linked immunosorbent assays (ELISA), and indirect immunofluorescence test were used for diagnosis of chikungunya fever, which are based on whole virus antigens. CHIKV E1, and E2 envelope proteins for the CHIKV-specific serodiagnostic reagents for chikungunya fever were expressed in baculovirus expression system. The seroreactivity of recombinant CHIKV E1 and E2 envelope proteins were evaluated using sera panels of patients from Laboratoire Marcel Merieux by indirect IgM capture ELISA. The recombinant CHIKV E1 and E2 envelope protein showed sensitivity of 77.5% and 90%, respectively. The specificities of both CHIKV E1 and E2 envelope proteins were 100%. The recombinant CHIKV E1 and E2 envelope proteins could be a useful diagnostic reagent for CHIKV infection.
影响因子：
2.4
作者：
Jin YH;Hong SH;Kim K;Shin HJ;Park S
通讯作者：
Park S