Detect ion of classical swine fever vaccine virus in blood and tissue samples of pigs vaccinated either with a conventional C-strain vaccine or a modified live marker vaccine
Detect ion of classical swine fever vaccine virus in blood and tissue samples of pigs vaccinated either with a conventional C-strain vaccine or a modified live marker vaccine
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DOI：
10.1016/j.vetmic.2006.10.034
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发表时间：
2007-03-10
影响因子：
3.3
通讯作者：
Beer, Martin
中科院分区：
文献类型：
--
作者：
Koenig, Patricia;Hoffmann, Bernd;Beer, Martin
猪瘟减毒活病毒是最有效的疫苗。然而，关于脑脊液疫苗病毒在宿主体内的分布和检测，人们知之甚少。因此，我们在疫苗接种后的前42天内，对新的重组市场减毒疫苗CP7_E2alf与传统的C株疫苗进行了病毒分离、抗原和基因组检测的比较。用高灵敏的实时逆转录聚合酶链式反应(RT-PCR)技术检测白细胞及扁桃体、淋巴结、脾、胸腺、腮腺和肾脏等脏器。结果表明，在免疫后14天内，只有极低滴度和基因组拷贝数的少数白细胞样本才能通过活体动物采样检测到疫苗病毒。在接种疫苗后的头6天内，也可以从扁桃体样本中分离到疫苗病毒。相比之下，通过实时RT-PCR，直到第42天，疫苗病毒基因组一直在扁桃体中检测到。两种测试疫苗的分布、病毒数量和病毒基因组水平相似。总之，即使在接种疫苗后14天以上的动物中，血液样本也可以作为检测脑脊液野生型病毒感染的首选样本，而扁桃体样本则为利用实时荧光RT-PCR方法长期检测两种被测脑脊液疫苗病毒提供了合适的材料。(C)2006爱思唯尔B.V.保留所有权利。
Attenuated live classical swine fever (CSF) viruses are the most efficacious vaccines against the disease. However, little is known about the distribution and detection of CSF vaccine viruses in the host. We therefore compared the new recombinant attenuated market- vaccine virus CP7_E2alf with the conventional C-strain vaccine concerning virus isolation, antigen-, and genome-detection in different samples within the first 42 days post-vaccination (p.v.). Leukocytes and several organs such as tonsils, lymph nodes, spleen, thymus, parotis and kidney were also tested using highly sensitive real-time reverse transcriptionpoiymerase chain reaction (RT-PCR) techniques. It was demonstrated that vaccine virus could be detected by live animal sampling only in a few leukocytes samples at very low titres and genome copy numbers within the first 14 days after iminunisation. Vaccine virus could also be isolated from individual tonsil samples within the first 6 days after vaccine application. In contrast, vaccine virus genomes were consistently detected in the tonsils up to day 42 by real-time RT-PCR. Distribution, amount of virus and viral genome levels were similar for both tested vaccines. In conclusion, blood samples could be the sample material of choice for detecting CSF wild type virus infection even in vaccinated animals after more than 14 days p.v., while tonsil sampling provided appropriate material for long-term detection of both tested CSF vaccine viruses using real-time RT-PCR methods. (c) 2006 Elsevier B.V. All rights reserved.