A plasmid which can be transferred between Escherichia coli and Pasteurella haemolytica by electroporation and conjugation.
A plasmid which can be transferred between Escherichia coli and Pasteurella haemolytica by electroporation and conjugation.
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一种可以通过电穿孔和接合在大肠杆菌和溶血巴斯德氏菌之间转移的质粒。
DOI：
10.1099/00221287-135-11-2885
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
N. Gilmour
中科院分区：
文献类型：
--
作者：
F. Craig;J. Coote;R. Parton;J. Freer;N. Gilmour
以溶血巴氏杆菌T179株(A1型)、Y216株(A2型)及其衣壳缺陷型变异体Y216/NS1为研究对象，用3个广宿主质粒(pRK290、pSa4和pKT230)和1个天然溶血巴氏杆菌质粒(PPH33)进行转化实验。用热休克和冻融技术都没有检测到转化子。然而，通过电穿孔，所有的溶血假单胞菌都能被pPH33转化，但不能被pRK290或pSA4转化。溶血假单胞菌Y216/NS1的转化子频率最高，为91×10(4)个转化子/微克。虽然pPH33本身不能通过接合传递，但可以利用IncP质粒pRK2013的转移功能，以0.3-2.2×10(-3)个/受体细胞的频率将其从大肠杆菌中动员到溶血巴氏杆菌中。
Three broad-host-range plasmids (pRK290, pSa4 and pKT230) and one native Pasteurella haemolytica plasmid (pPH33) were used in transformation experiments with P. haemolytica strains T179 (serotype A1), Y216 (serotype A2) and its capsular-deficient variant Y216/NS1. No transformants were detected with either heat-shock or freeze-thaw techniques. However, by electroporation, all P. haemolytica strains were transformed by pPH33 but not by pRK290 or pSa4. The highest frequency obtained was 91 x 10(4) transformants per microgram of pPH33 DNA with P. haemolytica strain Y216/NS1. Although pPH33 itself was non-transmissible by conjugation, it could be mobilized from Escherichia coli, using the transfer function of the IncP plasmid pRK2013, into P. haemolytica at a frequency of 0.3-2.2 x 10(-3) per recipient cell.
DOI：
10.1073/pnas.85.3.856
发表时间：
1988-02-01
影响因子：
11.1
作者：
MILLER, JF;DOWER, WJ;TOMPKINS, LS
通讯作者：
TOMPKINS, LS