Effects of gonadotropin-releasing hormone pulse frequency modulation on the reproductive axis of photoinhibited male Siberian hamsters.
Effects of gonadotropin-releasing hormone pulse frequency modulation on the reproductive axis of photoinhibited male Siberian hamsters.
复制标题
促性腺激素释放激素脉冲频率调制对光抑制雄性西伯利亚仓鼠生殖轴的影响。
DOI：
10.1095/biolreprod59.4.813
复制
发表时间：
1998
影响因子：
3.6
通讯作者：
Levine,JE
中科院分区：
文献类型：
--
作者：
Meredith,JM;Turek,FW;Levine,JE
在西伯利亚仓鼠中，光刺激引起促性腺激素的不同释放，FSH迅速上升，LH水平上升得晚得多。我们已经测试了这一假设，即该物种中促性腺激素的差异释放可以通过脉冲性GnRH刺激频率的变化来调节。光抑制西伯利亚仓鼠以每45分钟（快）、90分钟（中）或180分钟（慢）1次脉冲的频率接受GnRH脉冲。分别于治疗后0、3、5、10、20、30天处死。GnRH脉冲频率对LH释放有明显的影响，有快脉冲>中脉冲>慢脉冲>短日（SD）对照。此外，10天的快速GnRH脉冲产生的LH水平显著高于10天中速或慢速GnRH脉冲产生的LH水平。脉动型GnRH产生的血清FSH关系如下：中脉冲>快速脉冲> SD。FSH对较慢GnRH频率的反应介于两个较快频率之间。GnRH脉冲频率对配对睾丸体重的影响如下：快脉冲=中脉冲>慢脉冲> SD对照。不同的GnRH脉冲频率产生以下睾酮关系；快脉冲>中脉冲=慢脉冲= SD控制。这些结果与研究结果一致，研究表明较慢的GnRH脉冲频率促进FSH释放，而较快的GnRH脉冲频率有利于LH释放。我们的观察结果也与以下观点一致：仓鼠转入长日光周期后，FSH的单一释放是由内源性脉动性GnRH释放频率的改变介导的。
In Siberian hamsters, photostimulation evokes differential release of the gonadotropins, with FSH rising rapidly and LH levels rising much later. We have tested the hypothesis that differential release of gonadotropins in this species can be mediated by changes in the frequency of pulsatile GnRH stimulation. Photoinhibited Siberian hamsters received GnRH pulses at frequencies of 1 pulse every 45 (fast), 90 (medium), or 180 min (slow). Animals were killed at 0, 3, 5, 10, 20, and 30 days after treatment. There was a clear GnRH pulse frequency effect on LH release, with fast pulses > medium pulses > slow pulses > short-day (SD) controls. In addition, 10 days of fast-frequency GnRH pulses produced LH levels significantly greater than LH levels in animals exposed to 10 days of medium or slow GnRH pulse frequencies. Pulsatile GnRH produced the following serum FSH relationships: medium pulses > fast pulses > SD. The FSH response to slow GnRH frequency fell between the two faster frequencies. The effect of GnRH pulse frequency on paired testes weight was as follows: fast pulses = medium pulses > slow pulses > SD controls. The differing GnRH pulse frequencies produced the following testosterone relationships; fast pulses > medium pulses = slow pulses = SD controls. These results agree with studies showing that slower GnRH pulse frequencies facilitate FSH release, while faster GnRH pulse frequencies favor LH release. Our observations are also consistent with the idea that the singular release of FSH after transfer of hamsters to a long-day photoperiod is mediated by alterations in the frequency of endogenous pulsatile GnRH release.