Dietary Selenium Deficiency Exacerbates Lipopolysaccharide-Induced Inflammatory Response in Mouse Mastitis Models
Dietary Selenium Deficiency Exacerbates Lipopolysaccharide-Induced Inflammatory Response in Mouse Mastitis Models
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DOI：
10.1007/s10753-014-9925-y
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发表时间：
2014-05
期刊：
影响因子：
5.1
通讯作者：
Zhengkai Wei;Minjun Yao;Yimeng Li;Xuexiu He;Zhengtao Yang
中科院分区：
文献类型：
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作者：
Zhengkai Wei;Minjun Yao;Yimeng Li;Xuexiu He;Zhengtao Yang
硒 (Se) 是一种必需的微量营养素，在抗炎过程和抗氧化防御系统中发挥着关键作用。在这项研究中，我们在小鼠模型中研究了膳食缺硒对脂多糖（LPS）诱导的乳腺炎的影响。通过荧光原子吸收光谱法评估肝脏中的硒含量。按照相应试剂盒测定血液中谷胱甘肽过氧化物酶（GPx）活性、髓过氧化物酶（MPO）活性、乳腺组织上清液中肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-1β活性。通过蛋白质印迹法评估环加氧酶-2 (COX-2) 和诱导型一氧化氮合酶 (iNOS) 的表达。结果表明，成功复制了缺硒小鼠模型，在LPS诱导的小鼠乳腺炎中，缺硒加剧了乳腺组织病理学，增加了TNF-α和IL-1β的表达，并促进了iNOS和COX-2的激活。总之，我们的研究表明，缺硒会导致 LPS 诱导的小鼠乳腺炎发生更严重的炎症反应。
Selenium (Se) is an essential micronutrient that plays a critical role in anti-inflammatory processes and antioxidant defense system. In this study, we investigated the effects of dietary selenium deficiency on lipopolysaccharide (LPS)-induced mastitis in mouse models. Se content in the liver was assessed by fluorescent atomic absorption spectrometry. Glutathione peroxidase (GPx) activity in the blood, myeloperoxidase (MPO) activity, tumor necrosis actor alpha (TNF-α), and interleukin (IL)-1β in the supernatant of the mammary tissue were determined according to the corresponding kits. Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expressions were evaluated by Western blotting. The results showed that the Se-deficient mouse model was successfully replicated, and selenium deficiency exacerbated mammary gland histopathology, increased the expressions of TNF-α and IL-1β, and facilitated the activation of iNOS and COX-2 in LPS-induced mouse mastitis. In conclusion, our studies demonstrated that selenium deficiency resulted in more severe inflammatory response in LPS-induced mouse mastitis.