The use of repetitive DNA in cytogenetic studies of plant sex chromosomes
The use of repetitive DNA in cytogenetic studies of plant sex chromosomes
复制标题
DOI：
10.1159/000121074
复制
发表时间：
2008-01-01
影响因子：
1.7
通讯作者：
Matsunaga, S.
中科院分区：
文献类型：
--
作者：
Kazama, Y.;Matsunaga, S.
利用重复DNA进行荧光原位杂交（FISH）分析了植物性染色体的结构。从显微切割的性染色体扩增的DNA文库中成功获得了FISH探针。一些探针与亚端粒区域杂交，该区域存在多种重复DNA，其重复单元在染色体内具有相似性，而非染色体间相似性。例如，利用亚端粒重复序列进行的FISH能够轻易显示出蝇子草（Silene latifolia）X染色体上的拟常染色体区域（PAR）的位置。其他探针则定位在性染色体的中间区域。中间区域包含叶绿体DNA或内部端粒的邻近序列，这表明在性染色体分化过程中发生了插入或易位。这些数据对于理解植物性染色体的结构及其进化过程非常有价值。版权所有（c）2008 S. Karger AG，巴塞尔。
The structure of sex chromosomes in plants was analyzed by fluorescent in situ hybridization (FISH) with repetitive DNAs. FISH probes were successfully obtained from DNA libraries that were amplified from micro-dissected sex chromosomes. Some probes hybridized to the subtelomeric regions, where many kinds of repetitive DNAs are located with intrachromosomal similarity of their repeat units rather than interchromosomal similarity. For example, FISH with the subtelomeric repetitive sequence can easily show the location of the pseudoautosomal region PAR) on the X chromosome of Silene latifolia. The other probes were localized on the interstitial region of the sex chromosomes. The interstitial region contains chloroplast DNAs or neighboring sequences of the internal telomeres, suggesting insertion or translocation occurred during differentiation of the sex chromosome. These data are very informative for understanding the structure of the plant sex chromosomes and their evolutionary process. Copyright (c) 2008 S. Karger AG, Basel.