Dietary combination of fucoxanthin and fish oil attenuates the weight gain of white adipose tissue and decreases blood glucose in obese/diabetic KK-Ay mice
Dietary combination of fucoxanthin and fish oil attenuates the weight gain of white adipose tissue and decreases blood glucose in obese/diabetic KK-Ay mice
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DOI：
10.1021/jf071569n
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发表时间：
2007-09-19
影响因子：
6.1
通讯作者：
Miyashita, Kazuo
中科院分区：
文献类型：
--
作者：
Maeda, Hayato;Hosokawa, Masashi;Miyashita, Kazuo
岩藻黄质是一种在可食用的褐色海藻中发现的海洋类胡萝卜素。我们先前报道了膳食岩藻黄质减弱糖尿病/肥胖KK-A（y）小鼠的白色脂肪组织（WAT）的体重增加。在这项研究中，为了评估岩藻黄素和鱼油的抗肥胖和抗糖尿病作用，我们研究了对KK-Ay小鼠的WAT体重、血糖和胰岛素水平的影响。同时检测WAT中解偶联蛋白1（UCP 1）和脂肪因子mRNA的表达水平。喂食4周后，与对照小鼠相比，饲料中0.2%岩藻黄质显著减弱了KK-Ay小鼠WAT体重的增加，并增加了UCP 1表达。喂食0.1%岩藻黄质和6.9%鱼油的小鼠的WAT重量也显著低于仅喂食岩藻黄质的小鼠。此外，0.2%岩藻黄质显著降低KK-Ay小鼠的血糖和血浆胰岛素浓度。用0.1%岩藻黄质和鱼油的组合饮食喂养的小鼠也显示出与0.2%岩藻黄质相似的改善。0.2%岩藻黄质显著下调WAT中瘦素和肿瘤坏死因子（TNF α）mRNA的表达。这些结果表明，膳食岩藻黄质降低KK-Ay的血糖和血浆胰岛素浓度，沿着下调TNF α mRNA。此外，岩藻黄质和鱼油的组合比单独用岩藻黄质喂养更有效地减弱WAT的体重增加。
Fucoxanthin is a marine carotenoid found in edible brown seaweeds. We previously reported that dietary fucoxanthin attenuates the weight gain of white adipose tissue (WAT) of diabetic/obese KK-A(y) mice. In this study, to evaluate the antiobesity and antidiabetic effects of fucoxanthin and fish oil, we investigated the effect on the WAT weight, blood glucose, and insulin levels of KK-Ay mice. Furthermore, the expression level of uncoupling protein 1 (UCP1) and adipokine mRNA in WAT were measured. After 4 weeks of feeding, 0.2% fucoxanthin in the diet markedly attenuated the gain of WAT weight in KK-Ay mice with increasing UCP1 expression compared with the control mice. The WAT weight of the mice fed 0.1% fucoxanthin and 6.9% fish oil was also significantly lower than that of the mice fed fucoxanthin alone. In addition, 0.2% fucoxanthin markedly decreased the blood glucose and plasma insulin concentrations in KK-Ay mice. The mice fed with the combination diet of 0.1% fucoxanthin and fish oil also showed improvements similar to that of 0.2% fucoxanthin. Leptin and tumor necrosis factor (TNF alpha) mRNA expression in WAT were significantly down-regulated by 0.2% fucoxanthin. These results suggest that dietary fucoxanthin decreases the blood glucose and plasma insulin concentration of KK-Ay along with down-regulating TNFa mRNA. In addition, the combination of fucoxanthin and fish oil is more effective for attenuating the weight gain of WAT than feeding with fucoxanthin alone.