Expression analysis and characterization of an autosome-localized tesk1 gene in half-smooth tongue sole (Cynoglossus semilaevis).
Expression analysis and characterization of an autosome-localized tesk1 gene in half-smooth tongue sole (Cynoglossus semilaevis).
复制标题
DOI：
10.1016/j.gene.2016.02.010
复制
发表时间：
2016-05
期刊：
影响因子：
3.5
通讯作者：
Wen‐teng Xu;Hailong Li;Ning Zhang;Zhongdian Dong;Na Wang;C. Shao;Songlin Chen
中科院分区：
文献类型：
--
作者：
Wen‐teng Xu;Hailong Li;Ning Zhang;Zhongdian Dong;Na Wang;C. Shao;Songlin Chen
睾丸特异性蛋白激酶1（Testis-specific protein kinase 1，tesk 1）是一个保守的基因家族，在许多细胞过程中发挥作用。本研究克隆并鉴定了半滑舌鳎常染色体定位的tesk 1基因（Altesk 1），其开放阅读框由2088个核苷酸组成，编码665个氨基酸。系统发育分析表明，脊椎动物Tesk 1基因根据蛋白质长度分为两类，AlTesk 1属于“长型”类群。半定量PCR结果显示，Altesk 1主要在卵巢中表达，而在其他组织中的表达量相对较低。Altesk 1转录本仅在210日龄和1龄鱼的卵巢中发现，原位杂交进一步证实了其主要定位于卵母细胞中。比较不同性别基因型中Altesk 1基因启动子甲基化模式发现，Altesk 1基因启动子在女性中甲基化水平较低，这与Altesk 1基因在女性中高表达水平相一致。综上所述，这是首次发现tesk 1基因表现出雌性偏好性表达，鉴于此，我们推测AlTesk 1可能参与卵巢特异性的某些细胞过程，例如卵子发生。
Testis-specific protein kinase 1 (tesk1) represents a conserved gene family functioning in many cellular processes. In this study, we cloned and characterized an autosome-localizedtesk1gene (Altesk1) from Cynoglossus semilaevis.The open reading frame consists of 2088 nucleotides and encodes a 665 amino acid polypeptide. Phylogenetic analyses show that vertebrate Tesk1s are divided into two clusters based on protein length and AlTesk1 belongs to “long-type” group. Semi-quantitative PCR reveals thatAltesk1is predominantly expressed in ovary, despite of relatively low detection in some other tissues. Among different development stages,Altesk1transcripts are only observed in ovary samples of 210-day and 1-year fish.In situhybridization analyses have further confirmed its majorlocalization in oocyte cells. Comparison of methylation patterns in different sexual genotypes reveals the low methylation level ofAltesk1promoter in female, which is consistent withAltesk1high expression level in female. Taken together, this is the first time thattesk1gene has been found to show female-biased expression and in view of this, we postulate that AlTesk1 might be involved in some cellular processes specific in ovary, e.g. oogenesis.