Sex-specific modulation of anxiety and locomotion after neonatal exposure to pregnenolone sulfate.
Sex-specific modulation of anxiety and locomotion after neonatal exposure to pregnenolone sulfate.
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新生儿接触孕烯醇酮硫酸盐后焦虑和运动的性别特异性调节。
DOI：
10.1016/j.physbeh.2004.09.017
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Cruz,NelsonD
中科院分区：
文献类型：
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作者：
Jorge,JuanCarlos;Gonzalez,Loida;Fortis,Azarhi;Cruz,NelsonD
神经类固醇可能从发育早期就影响成人的行为和内分泌功能。为了验证这一假设，从出生后第1天到第14天，雌性SD幼崽都被注射了孕烯醇酮硫酸盐(PrEG-S)。雌性幼崽的青春期开始是通过监测阴道开放的日期来确定的。阴道开放后，连续监测3个周期的发情周期。在成年期间，焦虑和运动活动分别用自动高架迷宫(EPM)和自动活动监测器进行评估。在行为评估后，测量体重、性腺重量和血浆性激素水平。新生儿接触孕酮-S对女性产生了缓解焦虑的作用，但对男性没有影响。在男性身上看到了相反的效果，在不改变焦虑的情况下抑制了运动行为。成年动物的阴道开放日、性腺重量和血浆激素水平不受新生儿治疗的影响。我们认为，在新生儿发育期间接触孕酮-S可以以一种性别特异性的方式影响成年后的焦虑和运动行为。
Neurosteroids may influence adult behavior and endocrine function since early in development. To test this hypothesis, Sprague–Dawley pups from both sexes were injected with pregnenolone sulfate (PREG-S) from postnatal (PN) day 1 to 14. Pubertal onset in females was determined by monitoring the day of vaginal opening. After vaginal opening, the pattern of estrous cycle was monitored for three consecutive cycles. During adulthood, anxiety and locomotor activity were assessed with an automated elevated plus maze (EPM) and automated activity monitors, respectively. After behavioral assessment, body weight, gonadal weight, and plasma levels of sex hormones were measured. Neonatal exposure to PREG-S produced anxiolytic effects in females but not males. The opposite effect was seen in males, suppression of locomotor behaviors without changes in anxiety. The day of vaginal opening, gonadal weight, and plasma levels of hormones in adult animals were not altered by neonatal treatment. We suggest that PREG-S exposure during neonatal development can have an impact in anxiety and locomotor behaviors during adulthood in a sex-specific manner.