Adiponectin Expression Is Induced by Vitamin E via a Peroxisome Proliferator-Activated Receptor (cid:1) -Dependent Mechanism
Adiponectin Expression Is Induced by Vitamin E via a Peroxisome Proliferator-Activated Receptor (cid:1) -Dependent Mechanism
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维生素 E 通过过氧化物酶体增殖物激活受体 (cid:1) 诱导脂联素表达 - 依赖性机制
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
M. Amiot
中科院分区：
文献类型：
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作者：
J. Landrier;E. Gouranton;Claire El Yazidi;C. Malezet;P. Balaguer;P. Borel;M. Amiot
脂联素是一种众所周知的脂肪因子，由脂肪细胞分泌，具有胰岛素增敏特性。微量营养素对这种脂肪因子表达的调节在很大程度上是未知的。我们通过过氧化物酶体增殖激活受体（cid:1） （PPAR (cid:1)）途径证实了脂联素表达在暴露于生育酚后诱导脂肪细胞的作用。维生素E强制喂养导致小鼠在mRNA和蛋白质水平上诱导脂联素。维生素E (（cid:2) -和(cid:1) -生育酚）也增加了3T3-L1细胞脂联素mRNA和蛋白质的分泌，PPAR (cid:1) mRNA也增加，独立于抗氧化作用。（cid:2）和（cid:1）维生素的瞬时转染通过apr响应元件在人脂联素促进子的控制下诱导了荧光素的普遍表达。生育酚诱导脂联素似乎是PPAR （cid:1）依赖性的，因为它被特异性拮抗剂GW9662阻断。最后，我们发现PPAR （cid:1）内源性配体15-脱氧- (cid:1) 12,14-前列腺素J2的细胞内浓度在toco- pherolsin3t3 - l1细胞处理后增加。综上所述，维生素i上调脂联素表达的机制涉及PPAR （cid:1）及其内源性配体15-脱氧- (cid:1) 12,14-前列腺素J2。通过原始分子机制诱导脂联素可被认为是维生素E对胰岛素敏感性有益作用的基础。（内分泌学150:5318-5325,2009）一式三次，独立重复至少两次。
Adiponectin is a well-known adipokine secreted by adipocytes that presents insulin-sensitizing properties. The regulation of expression of this adipokine by micronutrients is largely unknown. We dem-onstrateherethatadiponectinexpressionisinducedinadipocytesafterexposuretotocopherolsviathe peroxisome proliferator-activated receptor (cid:1) (PPAR (cid:1) ) pathway. Vitamin E force feeding resulted in an induction of adiponectin in mice at both mRNA and protein levels. Adiponectin mRNA and protein secretion were also increased by vitamin E ( (cid:2) - and (cid:1) -tocopherol) in 3T3-L1 cells, together with PPAR (cid:1) mRNA,independentofanantioxidanteffect.Intransienttransfections,both (cid:2) -and (cid:1) -vitamersinduced theluciferasegenereporterunderthecontrolofahumanadiponectinpromoterviaaPPAR-responsive element. The induction of adiponectin by tocopherols seems to be PPAR (cid:1) dependent, because it was blocked by the specific antagonist GW9662. Finally, we showed that intracellular concentrations of a PPAR (cid:1) endogenous ligand, 15-deoxy- (cid:1) 12,14-prostaglandin J2, increased after treatment with toco-pherolsin3T3-L1cells.Insummary,vitaminEup-regulatesadiponectinexpressionviaamechanismthat implicates PPAR (cid:1) together with its endogenous ligand 15-deoxy- (cid:1) 12,14-prostaglandin J2. The induction of adiponectin via an original molecular mechanism could be considered as the basis for the beneficial effect of vitamin E on insulin sensitivity. ( Endocrinology 150: 5318–5325, 2009) The performed in triplicate and repeated at least two times independently.
影响因子：
16.2
作者：
Mayer-Davis, EJ;Costacou, T;Bell, RA
通讯作者：
Bell, RA