Muscle infections in imported neon tetra, Paracheirodon innesi myers: limited occurrence of microsporidia and predominance of severe forms of columnaris disease caused by an Asian genomovar of Flavobacterium columnare
Muscle infections in imported neon tetra, Paracheirodon innesi myers: limited occurrence of microsporidia and predominance of severe forms of columnaris disease caused by an Asian genomovar of Flavobacterium columnare
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进口霓虹灯四、Paracheirodon innesi myers 的肌肉感染：微孢子虫的发生率有限，并且由亚洲柱状黄杆菌基因型引起的严重形式的柱状菌病占主导地位
DOI：
10.1046/j.1365-2761.2002.00364.x
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发表时间：
2002
影响因子：
2.5
通讯作者：
J. Bernardet
中科院分区：
文献类型：
--
作者：
C. Michel;S. Messiaen;J. Bernardet
最近，法国观赏鱼贸易商在进口霓虹灯鱼（Paracheirodon innesi Myers）中发生了严重且反复的死亡事件，根据临床症状，根据经验将其归因于微孢子虫 Pleistophora hyph-essobryconis。虽然Pleistophora sp。少数病例观察到孢子，实验室结果表明，类似的临床症状一般是由致病菌柱状黄杆菌引起的。在所有情况下，肌肉是主要目标组织，最明显的外部迹象仅限于肤色褪色和白色坏死区域的发展。通过细菌学测试和分子技术对三种分离株进行了研究和分型。尽管它们的表型特征与 F.columnare 的描述一致，但除了较高的最佳生长温度（18-30°C）外，它们似乎在遗传上都与常见的欧洲和美洲分离株不同，并且与日本工作人员最近分配给新基因变种的亚洲分离株相似。实验感染表明这些分离株对观赏物种具有高毒力。讨论了使用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）来识别和检测组织样本中的病原体，以及这一发现对进口鱼类和国内物种健康控制管理的影响。
Serious and repeated mortality recently experienced in imported neon tetra, Paracheirodon innesi Myers, by French ornamental fish traders and empirically ascribed to the microsporidian Pleistophora hyph-essobryconis on the basis of clinical signs, was investigated. Although Pleistophora sp. spores were observed in a few cases, laboratory results demonstrated that similar clinical signs were generally caused by the pathogenic bacterium Flavobacterium columnare. In all cases, muscle was the main target tissue, and the most noticeable external signs were limited to fading of skin colouration and the development of white areas of necrosis. Three isolates were studied and typed by bacteriological tests and molecular techniques. Although their phenotypic characteristics were in accordance with F. columnare descriptions, except for higher optimal growth temperatures (18-30 °C), they all appeared to differ genetically from common European and American isolates and to be similar to Asian isolates recently assigned to a new genomovar by Japanese workers, Experimental infections suggested the isolates were highly virulent for ornamental species. The use of polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) for identification and detection of the agent in tissue samples, and the implications of this finding for health control management of imported fish and domestic species are discussed.