BASE-STACKING AND BASE-PAIRING CONTRIBUTIONS TO HELIX STABILITY - THERMODYNAMICS OF DOUBLE-HELIX FORMATION WITH CCGG, CCGGP, CCGGAP, ACCGGP, CCGGUP, AND ACCGGUP
BASE-STACKING AND BASE-PAIRING CONTRIBUTIONS TO HELIX STABILITY - THERMODYNAMICS OF DOUBLE-HELIX FORMATION WITH CCGG, CCGGP, CCGGAP, ACCGGP, CCGGUP, AND ACCGGUP
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DOI：
10.1021/bi00271a004
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发表时间：
1983-01-01
期刊：
影响因子：
2.9
通讯作者：
TURNER, DH
中科院分区：
文献类型：
--
作者：
PETERSHEIM, M;TURNER, DH
用分光光度法测定了CCGG、CCGGp、CCGGAp、ACCGGp、CCGGUp和ACCGGUP在1M氯化钠溶液中形成双螺旋的热力学。结果表明，末端不成对碱基赋予了额外的双螺旋稳定性。与5‘’A或3‘’U相比，3‘’A对双螺旋的稳定作用更强，稳定性的提高是由于对双螺旋的形成有更有利的热变。CCGG、ACCGGp、CCGGUp和ACCGGUp的热力学比较表明，在决定ACCGGUp中AU末端碱基对稳定性方面，堆积作用比配对作用更重要。
The thermodynamics of double-helix formation in 1 M NaCl were measured spectrophotometrically for CCGG, CCGGp, CCGGAp, ACCGGp, CCGGUp and ACCGGUP. The results indicate additional double-helical stability is conferred by the terminal unpaired bases. The 3'' A stabilizes the double helix more than the 5'' A or the 3'' U. The increased stability is due to a more favorable enthalpy change for double-helix formation. Comparison of the thermodynamics for CCGG, ACCGGp, CCGGUp, and ACCGGUp indicates stacking interactions are somewhat more important than pairing interactions in determining the stability of the terminal AU base pairs in ACCGGUp.