Time-of-flight mass spectrometry with an electrospray ion beam.
Time-of-flight mass spectrometry with an electrospray ion beam.
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DOI：
10.1021/ac00042a011
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发表时间：
1992-09
影响因子：
7.4
通讯作者：
J. Boyle;C. Whitehouse
中科院分区：
文献类型：
--
作者：
J. Boyle;C. Whitehouse
电喷雾电离源已与反射式飞行时间质谱仪联用。通过将离子源垂直于无场漂移区放置，离子束的纵向能量分散已大幅降低，使得对于具有生物学意义的低质量和高质量离子都能实现超过1000的质量分辨率。此外，仪器的灵敏度能够常规检测低皮摩尔和亚皮摩尔量的大型多电荷物质，比如细胞色素c（分子量 = 12360.9）。鉴于该仪器的简单性、快速扫描速度和高灵敏度，本文讨论了其用于对色谱流出物进行快速筛选的潜在用途。
An electrospray ionization source has been coupled to a reflection time-of-flight mass spectrometer. By orienting the ion source perpendicular to the field-free drift region, the longitudinal energy spread of the ion packet has been substantially reduced, allowing a mass resolving power of over 1000 to be achieved for both low-mass and high-mass ions of biological interest. In addition, instrument sensitivity allows the routine detection of low-picomole and subpicomole quantities of large multiply charged species such as cytochrome c (MW = 12,360.9). The potential utility of this instrument for conducting rapid screening of chromatographic effluents is discussed in light of its simplicity, rapid scanning speed, and high sensitivity.