TaWRKY10 transcription factor is a novel jasmonic acid signalling regulator involved in immunity against Septoria tritici blotch disease in wheat
TaWRKY10 transcription factor is a novel jasmonic acid signalling regulator involved in immunity against Septoria tritici blotch disease in wheat
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DOI：
10.1111/ppa.13388
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发表时间：
2021-04
期刊：
影响因子：
2.7
通讯作者：
Alberto Campanaro;A. Srivastava;Cunjin Zhang;Jack Lee;Linda Millyard;A. Gatehouse;Ed Byrne;A. Sadanandom
中科院分区：
文献类型：
--
作者：
Alberto Campanaro;A. Srivastava;Cunjin Zhang;Jack Lee;Linda Millyard;A. Gatehouse;Ed Byrne;A. Sadanandom
小麦斑枯病 (STB) 由真菌小麦发酵病斑菌 (Zymoseptoria tritici) 引起，目前是全球温带气候地区小麦生产的主要威胁。了解支持 Z 的遗传机制。小麦与小麦的相互作用对于制定针对STB的新控制策略至关重要。植物激素是生长和免疫的重要调节剂。特别是，茉莉酸 (JA) 在防御坏死性植物病原体方面发挥着核心作用，但其在介导针对 Z 的免疫方面发挥着重要作用。小麦很大程度上是未知的。在这里，我们鉴定了转录因子 TaWRKY10，它在分类学上仅限于禾本科亚科 Pooideae，作为小麦 JA 反应的新型调节剂。我们通过使用病毒诱导的基因沉默证明，沉默 TaWRKY10 会导致对 Z 的抵抗力增加。小麦坏死症状较早出现，但病原体孢子形成减少。 TaWRKY10 沉默的小麦植株表现出上调的 JA 反应。 TaWRKY10 敲低植物的转录谱表明，它是 JA 受体 TaCOI1 基因表达的负调节因子。我们的数据表明 TaWRKY10 下调 JA 反应，导致小麦对 Z 的敏感性。小麦。我们假设操纵 TaWRKY10 可能提供一种增强小麦 STB 抗性的策略。
Septoria tritici blotch (STB), caused by the fungus Zymoseptoria tritici , is currently the main threat to worldwide wheat production in temperate climates. Understanding the genetic mechanisms that underpin the Z . tritici– wheat interaction will be crucial for generating new control strategies against STB. Plant hormones are essential regula tors of growth and immunity. In particular, jasmonic acid (JA) plays a central role in de fence against necrotrophic plant pathogens, but its role in mediating immunity against Z . tritici is largely unknown. Here we identify the transcription factor TaWRKY10 that is taxonomically restricted to the grass subfamily Pooideae as a novel regulator of JA responses in wheat. We demonstrate by using virus- induced gene silencing that silencing TaWRKY10 leads to increased resistance against Z . tritici with an earlier onset of necrotic symptoms, but with reduced pathogen sporulation. Wheat plants silenced for TaWRKY10 show an up- regulated JA response. Transcriptional profiling of TaWRKY10 knock- down plants indicates that it is a negative regulator of the JA receptor TaCOI1 gene expression. Our data indicate that TaWRKY10 down- regulates JA responses, contributing to the susceptibility of wheat to Z . tritici . We postulate that manipulating TaWRKY10 may provide a strategy to boost STB resistance in wheat.