A kinetic analysis of strand breaks on large DNA induced by cigarette smoke extract
A kinetic analysis of strand breaks on large DNA induced by cigarette smoke extract
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DOI：
10.1016/j.cplett.2010.05.028
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发表时间：
2010-06-17
影响因子：
2.8
通讯作者：
Mizuno, Akira
中科院分区：
文献类型：
--
作者：
Kurita, Hirofumi;Takata, Tatsuya;Mizuno, Akira
我们报告了香烟烟雾提取物（CSE），可溶性香烟烟雾成分的提取物诱导的大DNA分子链断裂的动力学分析。以前，这种DNA损伤是通过琼脂糖凝胶电泳分析的，而我们使用荧光动力学分析单个DNA分子的损伤。CSE引起DNA分子长度的显著变化。使用简单的理论模型确定CSE诱导的大随机卷曲DNA分子上的双链断裂的速率，允许对大DNA分子上的双链断裂的速率进行简单的估计。(C)2010年爱思唯尔B。版权所有© 2016
We report a kinetic analysis of strand breakages on large DNA molecules induced by cigarette smoke extract (CSE), an extract of soluble cigarette smoke components. Previously, this DNA damage was analyzed by agarose gel electrophoresis, whereas we used fluorescence to kinetically analyze damage to individual DNA molecules. CSE caused a marked change in length of DNA molecules. The rate of CSE-induced double-strand breakage on large random-coiled DNA molecules was determined using a simple theoretical model, allowing the facile estimation of the rate of double-strand breaks on large DNA molecules. (C) 2010 Elsevier B. V. All rights reserved.