Heterotrophic nitrification and aerobic denitrification by a novel groundwater origin cold-adapted bacterium at low temperatures
Heterotrophic nitrification and aerobic denitrification by a novel groundwater origin cold-adapted bacterium at low temperatures
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DOI：
10.1039/c4ra13141j
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发表时间：
2015
期刊：
影响因子：
3.9
通讯作者：
D. Qu;Cong Wang;Yanfang Wang;Rui Zhou;Hejun Ren
中科院分区：
文献类型：
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作者：
D. Qu;Cong Wang;Yanfang Wang;Rui Zhou;Hejun Ren
从受苯胺污染的地下水系统中分离到一株具有nirS和nosZ基因的冷适应细菌Pseudomonasmigulae（AN-1）。在10 °C有氧条件下，AN-1可以分别以1.56、1.57和0.69 mg L−1 h−1的速率去除铵、硝酸盐和亚硝酸盐。在非竞争性底物抑制下，硝酸盐去除遵循Monod动力学（k = 270.27 mg NO3−-N g X−1 h−1，KS = 43.65 mg L−1，KSI = 185 mg L−1）。氮平衡分析表明，AN-1的气态反硝化途径是通过亚硝酸盐转化为N2。此外，以pH、溶解氧和NO3−-N浓度为自变量，对AN-1在10 °C条件下去除硝酸盐的效果进行了优化，得到了具有统计学显著性（R2 = 0.9840，p < 0.0001）的二次多项式数学模型，显示了AN-1在低温条件下去除废水中氮的潜力。
The cold-adapted bacterium Pseudomonas migulae (AN-1), which has nirS and nosZ genes, was isolated from an aniline-contaminated groundwater system. AN-1 could remove ammonium, nitrate, and nitrite at 1.56, 1.57, and 0.69 mg L−1 h−1, respectively, at 10 °C under aerobic conditions. Nitrate removal followed Monod kinetics under a non-competitive substrate inhibition (k = 270.27 mg NO3−–N g X−1 h−1, KS = 43.65 mg L−1, and KSI = 185 mg L−1). The putative gaseous denitrification pathway in AN-1 was via nitrite to N2 using nitrogen balance assays. Moreover, the pH, dissolved oxygen, and NO3−–N concentration were used as independent variables for the optimization of nitrate removal by AN-1 at 10 °C, and a statistically significant (R2 = 0.9840, p < 0.0001) quadratic polynomial mathematical model was found. This study highlights the potential of AN-1 for nitrogen removal from wastewaters at low temperatures.