Effect of water temperature on protein requirement of Heteropneustes fossilis (Bloch) fry as determined by nutrient deposition, hemato-biochemical parameters and stress resistance response
Effect of water temperature on protein requirement of Heteropneustes fossilis (Bloch) fry as determined by nutrient deposition, hemato-biochemical parameters and stress resistance response
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根据养分沉积、血液生化参数和抗逆反应确定水温对化石异棘鱼苗蛋白质需求的影响
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发表时间：
2020
期刊：
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通讯作者：
I. Ahmed
中科院分区：
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作者：
Shabih Fatma;I. Ahmed
膳食蛋白质需求取决于多种因素，水温是影响鱼类蛋白质需求的最重要的非生物因素之一。因此，本研究旨在调查水温对化石异翅鱼苗膳食蛋白质需求的影响，该鱼苗在大多数亚洲市场都有很高的需求。每个处理组有 30 条鱼（2.97 ± 0.65 cm；5.11 ± 0.34 g），每组四组，喂食七种等能量饲料（17.9 kJ g−1 总能量；14.99 kJ g−1 可消化能量），在两种水温（18 和 26 °C）下，饲料蛋白质水平范围为 28% 至 52%。实验饮食以半湿蛋糕形式喂食，每天三次，时间为 17:00、12:00 和 17:30，持续 12 周。为了获得准确的信息，分析了各种生长参数、蛋白质沉积、血液学参数、代谢酶和应激反应，并根据上述参数的响应推荐水温对日粮蛋白质需求的影响。保持在 26°C 的组在 44% 日粮蛋白质下获得了最佳生长、饲料转化率和蛋白质沉积，表明温度影响了化石鱼苗最佳生长的日粮蛋白质需求，并且 44% 日粮蛋白质似乎可以满足蛋白质需求。有趣的是，没有发现膳食蛋白质水平和温度的交互作用（P > 0.05）。在18℃水温下饲养的鱼，其天冬氨酸和丙氨酸转移酶的值相对于在26℃水温下饲养的鱼而言较高，这些酶表现出正常的生理值，表明在该温度下身体代谢正常。血液学参数也遵循相同的模式。此外，在26°C水温下饲养的鱼表现出更强的耐热应激能力（P<0.05）。使用二次多项式回归分析得到的蛋白质沉积数据的 95% 最大平台表明，在 26 °C 水温下，化石化石鱼苗的膳食蛋白质需求量为膳食的 40.8% 至 41.8%。鱼苗的日粮蛋白质水平和蛋白质/消化能比的推荐范围分别为 40.8–41.8% 和 27.21–27.88 mg 蛋白质 kJ−1 可消化能。所开发的信息对于通过更好地利用 26°C 下的营养物质来优化生长潜力以及为这种高度受欢迎的鱼类的大规模养殖制定有效的管理策略具有重要意义。
Dietary protein requirements are dependent on a variety of factors and water temperature is one of the most important abiotic factors affecting protein requirement of fish. This study was, therefore, conducted to investigate effects of water temperature on dietary protein requirement of fry Heteropneustes fossilis which has high demand in most of the Asian markets. Quadruplicate groups of 30 fish per treatment (2.97 ± 0.65 cm; 5.11 ± 0.34 g) were fed seven isoenergetic diets (17.9 kJ g−1 gross energy; 14.99 kJ g−1 digestible energy) containing dietary protein levels ranging from 28 to 52% at two water temperatures (18 and 26 °C). Experimental diets were fed to apparent satiation as semi-moist cakes thrice daily at 17:00, 12:00, and 17:30 h for 12 weeks. For precise information, various growth parameters, protein deposition, hematological parameters, metabolic enzymes, and stress response were analyzed, and effects of water temperature on dietary protein requirement was recommended on the basis of response from above parameters. Groups held at 26°C attained best growth, feed conversion, and protein deposition at 44% dietary protein indicating that temperature affected dietary protein requirement for optimum growth of H. fossilis fry and protein requirement seems to be satisfied with 44% dietary protein. Interestingly, interactive effects of both dietary protein levels and temperature were not found (P > 0.05). Fish reared at 18 °C had comparatively higher values for aspartate and alanine transferases than those reared at 26 °C water temperature which exhibited normal physiological value for these enzymes indicating that body metabolism was normal at this temperature. Hematological parameters also followed same pattern. Furthermore, fish reared at 26 °C water temperature exhibited more resistant to thermal stress (P < 0.05). The 95% maximum plateau of protein deposition data using second-degree polynomial regression analyses exhibited dietary protein requirement of fry H. fossilis between 40.8 and 41.8% of diet at 26 °C water temperature. The recommended range of dietary protein level and protein/digestible energy ratio for fry H. fossilis is 40.8–41.8% and 27.21–27.88 mg protein kJ−1 digestible energy, respectively. Information developed is of high significance for optimizing growth potential by making better utilization of nutrient at 26 °C and, to develop effective management strategies for mass culture of this highly preferred fish species.