Effect of proanthocyanidin-rich extract from Pinus radiata bark on immune response of specific-pathogen-free White Leghorn chickens
Effect of proanthocyanidin-rich extract from Pinus radiata bark on immune response of specific-pathogen-free White Leghorn chickens
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DOI：
10.3382/ps.2010-01160
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发表时间：
2011-05-01
期刊：
影响因子：
4.4
通讯作者：
Jang, H. -K.
中科院分区：
文献类型：
--
作者：
Park, I. -J.;Cha, S. -Y.;Jang, H. -K.
原花青素是一种天然化合物，广泛存在于水果、蔬菜、坚果、种子、花朵和树皮中。研究了辐射松树皮原花青素提取物（PAE）对无致病菌白来角鸡的免疫调节作用。20 mg/kg PAE处理2周后，鸡外周血单个核细胞增殖显著增强。5、10和20 mg/kg PAE处理5周后，鸡脾细胞和法氏囊细胞的增殖显著增强。5、10 mg/kg PAE处理5周后，胸腺细胞增殖显著增强。脂多糖（作用于负责体液免疫的B细胞）和刀豆蛋白A（直接作用于参与细胞介导免疫的T细胞）的存在显著增强了这些作用。PAE显著促进T辅助1细胞因子（干扰素- γ）的表达，降低T辅助2细胞因子（IL-6）的表达。由此可见，辐射假单胞菌PAE对无特定病原体的白来客那鸡具有免疫调节作用。
Proanthocyanidins are naturally occurring compounds that are widely found in fruits, vegetables, nuts, seeds, flowers, and bark. We evaluated the immunomodulatory effects of proanthocyanidin-rich extract (PAE) from Pinus radiata bark in specific-pathogen-free White Leghorn chickens. Proliferation of peripheral blood mononuclear cells was significantly enhanced in chickens treated for 2 wk with 20 mg/kg of PAE. Proliferation of splenocytes and bursal cells was significantly enhanced in chickens treated for 5 wk with 5, 10, and 20 mg/kg of PAE. Thymocyte proliferation was significantly enhanced in chickens treated for 5 wk with 5 and 10 mg/kg of PAE. These effects were markedly enhanced by the presence of lipopolysaccharide, which acted on B cells responsible for humoral immunity, and concanavalin A, which acted directly on T cells involved in cell-mediated immunity. The PAE significantly promoted the expression of T helper 1 cytokine (interferon-gamma) and decreased the expression of T helper 2 cytokine (IL-6). Thus, P. radiata PAE has immunomodulatory effects in specific-pathogen-free White Leghorn chickens.