Biochemical and metabolic abnormalities in normal and osteoarthritic human articular cartilage.
Biochemical and metabolic abnormalities in normal and osteoarthritic human articular cartilage.
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DOI：
10.1002/art.1780270109
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发表时间：
1984
影响因子：
--
通讯作者：
J. Ryu;B. Treadwell;H. Mankin
中科院分区：
文献类型：
--
作者：
J. Ryu;B. Treadwell;H. Mankin
用人正常和骨关节炎关节软骨器官培养研究了放射性前体物掺入大分子中的情况。通过氯化铯浮力密度梯度离心分离的盐提取的基质组分的分析表明，从骨关节炎软骨制备的所有梯度级分的比活性增加。对这些组分的进一步分析表明，骨关节炎软骨中含有5倍于蛋白聚糖的硫酸盐，并且含有更大量的3 H-葡糖胺，这些3 H-葡糖胺被掺入到沉降到梯度中间的材料中。根据从DEAE柱洗脱的位置及其对透明质酸酶消化的敏感性判断，超过一半的放射性中间馏分似乎是透明质酸盐。这项研究支持早期的研究结果，显示骨关节炎中大分子合成速率增加，此外，还证明了透明质酸的合成速率更高。
Incorporation of radioactive precursors into macromolecules was studied with human normal and osteoarthritic articular cartilage organ culture. Analysis of the salt extracted matrix components separated by cesium chloride buoyant density gradient centrifugation showed an increase in the specific activities of all gradient fractions prepared from the osteoarthritic cartilage. Further analysis of these fractions showed the osteoarthritic cartilage contained 5 times as much sulfate incorporated into proteoglycans, and an even greater amount of 3H-glucosamine incorporated into material sedimenting to the middle of the gradient. Greater than half of this radioactive middle fraction appears to be hyaluronate, as judged by the position it elutes from a DEAE column and its susceptibility to hyaluronidase digestion. This study supports earlier findings showing increased rates of macromolecular synthesis in osteoarthritis, and in addition, an even greater synthetic rate for hyaluronic acid is demonstrated.