Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot-resistant cultivars of Chinese cabbage (Brassica rapa L. ssp pekinensis)
Variation in virulence of Plasmodiophora brassicae in Japan tested with clubroot-resistant cultivars of Chinese cabbage (Brassica rapa L. ssp pekinensis)
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DOI：
10.1023/a:1008705413127
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发表时间：
1999-06-01
影响因子：
1.8
通讯作者：
Hirai, M
中科院分区：
文献类型：
--
作者：
Kuginuki, Y;Yoshikawa, H;Hirai, M
Williams（1966）和European Clubroot differential (ECD) （Buczacki et al., 1975）的差异寄主已被普遍用于鉴定导致芸苔类作物中棍棒病的芸苔菌（Plasmodiophora brassicae）种群。然而，其中一些寄主对大多数来自日本的种群表现出中等和波动的分数。因此，这些宿主无法在日本提供明确的分类。本研究利用日本抗棒孢杆菌（CR） F-1杂种（F-1）品种和油菜品系，对日本油菜（Brassica rapa）致病性遗传多样性进行了研究。部分crf -1品种的反应非常明显。利用crf -1品种在日本鉴定了4个大田群体。这里得到的明确反应可能在很大程度上取决于F-1品种的遗传纯度。此外，使用威廉姆斯集和ECD 01到ECD 05，可以将这些日本人群中的一些划分为同一种族。目前的差异寄主可能对油菜科欧洲居群的研究有用。不同寄主的反应表明，rapa中有几个主要的CR基因。因此，对CR基因进行金字塔化将有助于培育CR品种，以克服目前大白菜CR品种的缺陷。
The differential hosts of Williams (1966) and the European Clubroot Differential (ECD) (Buczacki et al., 1975) have been used commonly to identify populations of Plasmodiophora brassicae, which causes clubroot disease in Brassica crops. However, some of these hosts showed intermediate and fluctuating scores to most populations from Japan. Therefore, these hosts could not be used to provide a clear classification in Japan. We have tried to clarify the genetic diversity in pathogenicity of P. brassicae in Japan using Japanese clubroot-resistant (CR) F-1 hybrid (F-1) cultivars and lines of Brassica rapa. The responses of some CR F-1 cultivars were very clear. Four groups of field populations in Japan were recognized using the CR F-1 cultivars. The clear response obtained here may depend largely on the genetic purity of the F-1 cultivars. Moreover, it is possible to classify some of these Japanese populations into the same race using the Williams set and ECD 01 to ECD 05. The present differential hosts may be useful in the study of European populations of P. brassicae. The response of the differential hosts suggests that there are several major CR genes in B. rapa. It is suggested that pyramiding CR genes would be useful in breeding CR cultivars that can overcome the breakdown of the present CR cultivars of Chinese cabbage.