Survival and number of olfactory ensheathing cells transplanted in contused spinal cord of rats
Survival and number of olfactory ensheathing cells transplanted in contused spinal cord of rats
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DOI：
10.3760/cma.j.issn.1008-1275.2010.06.007
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发表时间：
2010-12-01
影响因子：
2.1
通讯作者：
Li Bing-cang
中科院分区：
文献类型：
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作者：
Li Yue;Yu Hua-lin;Li Bing-cang
目的：观察嗅鞘细胞（olfactory ensheathing cells，OECs）移植于挫伤脊髓内的存活情况及数量，为进一步研究OECs的生物学作用提供依据。用NYU-impactor II在T-10水平通过从25 mm高度落下10 g棒挫伤大鼠脊髓。在损伤后1周，从大鼠的绿色荧光蛋白（GFP）新鲜分离的OECs被移植到损伤部位和距离尾侧和吻侧端1 mm的另外两个部位的脊髓中，OECs数量为30000个/μ l × 3 = 90000。移植后1 ~ 13周，荧光显微镜下定性和半定量观察嗅鞘细胞存活情况及数量变化。结果：GFP-OECs在损伤脊髓内可存活至少13周。伤后1 ~ 13周，其排列由紧到松，数量逐渐减少。第1周平均GFP-OECs数为536个，少于原代移植的1%。此后，GFP-OECs数量持续下降，但以1 ~ 2周下降最为明显。其他时间点的降低程度相对较轻。结论：不同动物移植后GFP-OECs的存活率和数量不同，受宿主脊髓病理反应的影响。它还与脊髓挫伤后运动功能的恢复有关。
Objective: To observe the survival and the number of olfactory ensheathing cells (OECs) transplanted in the contused spinal cord, so as to provide a basis for further studying the biological action of OECs.Methods: The rat spinal cords were contused with NYU-impactor II at T-10 level by dropping a 10 g rod from a height of 25 mm. At the 1st week after injury, OECs isolated freshly from green fluorecense protein (GFP) of the rats were transplanted into the spinal cord at injured site and other two sites 1 mm apart from the caudal and rostral ends with the OECs number of 30000/mu lx3 = 90000. The survival and the number of OECs were qualitatively and semi-quantitatively observed under the fluorescense microscope from 1 week to 13 weeks after transplantation. The motor function of the cord was evaluated with BBB score.Results: GFP-OECs could survive at least for 13 weeks within the contused spinal cord. Their arrangement was from tight to loose and their number was decreased from 1 week to 13 weeks after injury. The average number of GFP-OECs was 536 at the 1st week, which was less than 1% of the number as compared with original transplantation. After then, the number of GFP-OECs was continually decreased, but the most obvious decrease was found during 1 week to 2 weeks. The extent of decrease at other time points was relatively mild. In contrast to the cell number, motor function of the cord was gradually recovered after transplantation.Conclusions: The survival and the number of GFP-OECs are different between the animals and are affected by the pathological reaction of the host cord. Also it is related to the motor function recovery of the contused cord.