Characterization of Tn3000, a Transposon Responsible for blaNDM-1 Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India
Characterization of Tn3000, a Transposon Responsible for blaNDM-1 Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India
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DOI：
10.1128/aac.01458-15
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发表时间：
2015-12-01
影响因子：
4.9
通讯作者：
Mello Sampaio, Jorge Luiz
中科院分区：
文献类型：
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作者：
Campos, Juliana Coutinho;Felix da Silva, Maria Jose;Mello Sampaio, Jorge Luiz
在肠杆菌科中，BLA(NDM)基因在许多不同的遗传环境中被发现，并发现了大量携带这些基因的载体。2013年8月，我们从一名在巴西里约热内卢住院的患者的直肠拭子样本中鉴定出产生NDM-1的大肠杆菌和霍尔马切肠杆菌，该患者没有出国旅行的历史。利用Illumina平台完成DNA测序，并对含有blA(NDM-1)基因的两个质粒(每个菌株一个)进行注释，结果表明它们属于不亲和组IncFII(K)和IncX3，并含有一个新的转座子Tn3000。在巴西的其他分离株中也发现了类似的遗传结构，但在其他大陆的质粒上也发现了类似的遗传结构。我们的发现表明，BLA(NDM-1)基因可能由Tn3000在世界不同地区传播。
In Enterobacteriaceae, the bla(NDM) genes have been found in many different genetic contexts, and a wide diversity of plasmid scaffolds bearing those genes has been found. In August 2013, we identified NDM-1-producing Escherichia coli and Enterobacter hormaechei strains from a single rectal swab sample from a patient hospitalized in Rio de Janeiro, Brazil, who had no history of travel abroad. Complete DNA sequencing using the Illumina platform and annotation of the two plasmids harboring the bla(NDM-1) gene, one from each strain, showed that they belonged to incompatibility groups IncFII(K) and IncX3 and harbored a novel transposon named Tn3000. Similar genetic structures have been identified among other isolates in Brazil but also on plasmids from other continents. Our findings suggest that the bla(NDM-1) gene may be transmitted by Tn3000 in different parts of the world.