Development of an Animal Model for Radiofrequency Ablation of Primary, Virally Induced Hepatocellular Carcinoma in the Woodchuck
Development of an Animal Model for Radiofrequency Ablation of Primary, Virally Induced Hepatocellular Carcinoma in the Woodchuck
复制标题
DOI：
10.1016/j.jvir.2011.08.020
复制
发表时间：
2011-11-01
影响因子：
2.9
通讯作者：
Fuchs, Henry
中科院分区：
文献类型：
--
作者：
Burke, Charles T.;Cullen, John M.;Fuchs, Henry
目的：目的：建立一种稳定可靠的射频消融治疗原发性肝细胞癌（HCC）的动物模型。材料与方法：15只土拨鼠接种土拨鼠肝炎病毒（WHV）建立慢性感染模型。当血清γ-谷氨酰转肽酶水平升高时，对动物进行超声评估，在大多数情况下，术前磁共振（MR）成像以确认肿瘤的发展。最后，通过使用1 cm探头对肿瘤进行射频消融，动物浸没在水浴中接地。结果：15只土拨鼠接受了射频消融治疗。对初始研究设计进行了修改，以适应土拨鼠的方法。这些动物中的最后10只用标准化方案治疗，使用1 cm探针产生一致的肿瘤坏死区域（平均消融尺寸，10.2 mm x 13.1 mm），无并发症。开发了一种安全、可靠和一致的方法来研究使用慢性WHV感染的土拨鼠进行自发性原发性HCC的射频消融，一种B型肝炎病毒诱导的肝癌动物模型。
Purpose: To develop a consistent and reproducible method in an animal model for studies of radiofrequency (RF) ablation of primary hepatocellular carcinoma (HCC).Materials and Methods: Fifteen woodchucks were inoculated with woodchuck hepatitis virus (WHV) to establish chronic infections. When serum gamma-glutamyl transpeptidase levels became elevated, the animals were evaluated with ultrasound, and, in most cases, preoperative magnetic resonance (MR) imaging to confirm tumor development. Ultimately, RF ablation of tumors was performed by using a 1-cm probe with the animal submerged in a water bath for grounding. Ablation effectiveness was evaluated with contrast-enhanced MR imaging and gross and histopathologic analysis.Results: RF ablation was performed in 15 woodchucks. Modifications were made to the initial study design to adapt methodology for the woodchuck. The last 10 of these animals were treated with a standardized protocol using a 1-cm probe that produced a consistent area of tumor necrosis (mean size of ablation, 10.2 mm x 13.1 mm) and led to no complications.Conclusions: A safe, reliable and consistent method was developed to study RF ablation of spontaneous primary HCC using chronically WHV-infected woodchucks, an animal model of hepatitis B virus induced HCC.