Gastrin induction of histamine release from primary cultures of canine oxyntic mucosal cells.
Gastrin induction of histamine release from primary cultures of canine oxyntic mucosal cells.
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胃泌素诱导犬泌酸粘膜细胞原代培养物释放组胺。
DOI：
10.1152/ajpgi.1992.263.4.g460
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Soll,AH
中科院分区：
文献类型：
--
作者：
Chuang,CN;Tanner,M;Chen,MC;Davidson,S;Soll,AH
使用酶分散的犬泌酸粘膜细胞，我们研究了组胺释放的调节，其中肥大细胞在很大程度上被去除的密度梯度的馏分。组胺样免疫反应性表现为过氧化物酶-抗过氧化物酶免疫组化。通过白蛋白阶梯密度梯度进一步分离小细胞淘析器组分（SCEF）中的含组胺细胞。低密度部分（LDF）中约2.5%的细胞含有组胺样免疫反应性;该部分基本上耗尽了更致密的肥大细胞（0.5%）。将这两种级分在基质胶基质上培养48-64小时。放射酶法测定细胞内组胺含量及释放量。胃泌素，卡巴胆碱，毛喉素增加组胺释放的LDF。胃泌素对组胺释放的诱导作用在5分钟内明显，并持续至少60分钟。在10（-11）和10（-8）M浓度之间，对胃泌素的反应呈剂量依赖性。与此相反，在肥大细胞富集SCEF，基础释放较高，胃泌素是没有影响，但是，刀豆球蛋白A刺激和肾上腺素抑制组胺释放表明组胺释放机制是完整的，在这部分。我们的方法提供了低密度泌酸粘膜组胺细胞的制备，这些细胞表现出胃泌素反应性组胺释放;我们推测肠嗜铬样细胞解释了这种胃泌素反应。
Using enzyme-dispersed canine oxyntic mucosal cells, we studied regulation of histamine release from fractions in which mast cells were largely removed by density gradient. Histamine-like immunoreactivity was demonstrated using peroxidase-anti-peroxidase immunohistochemistry. Histamine-containing cells in the small cell elutriator fractions (SCEF) were further separated by albumin step density gradients. Approximately 2.5% of cells in the low density fraction (LDF) contained histamine-like immunoreactivity; this fraction was largely depleted of the more dense mast cells (0.5%). These two fractions were cultured for 48-64 h on a Matrigel substrate. The cell content of histamine and release into the medium were measured by radioenzymatic assay. Gastrin, carbachol, and forskolin increased histamine release from the LDF. The induction of histamine release by gastrin was evident within 5 min and was sustained for at least 60 min. The response to gastrin was dose dependent between concentrations of 10(-11) and 10(-8) M. In contrast, in the mast cell-enriched SCEF, basal release was higher and gastrin was without effect; however, concanavalin A stimulated and epinephrine inhibited histamine release indicating that histamine-release mechanisms were intact in this fraction. Our methods provide a preparation of low density oxyntic mucosal histamine cells that demonstrate gastrin-responsive histamine release; we speculate that enterochromaffin-like cells account for this gastrin response.