Placental extracellular matrix: Gene expression, deposition by placental fibroblasts and the effect of oxygen
Placental extracellular matrix: Gene expression, deposition by placental fibroblasts and the effect of oxygen
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DOI：
10.1053/plac.2002.0904
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发表时间：
2003-04-01
期刊：
影响因子：
3.8
通讯作者：
Aplin, JD
中科院分区：
文献类型：
--
作者：
Chen, CP;Aplin, JD
数据库挖掘揭示了人胎盘中表达的约 10 000 个 mRNA 种类中的 102 个细胞外基质 (ECM) 基因，这些基因分为胶原蛋白 (23)、非胶原糖蛋白 (59) 和蛋白聚糖 (23)。使用一组针对选定胶原蛋白和糖蛋白的抗体来检查胎盘绒毛基质中的 ECM 分布。 I 型和 IV 型胶原蛋白、纤连蛋白和原纤维蛋白 I 在妊娠早期和足月组织中含量丰富。一些区域缺乏胶原蛋白 1，而胶原蛋白 IV 在间质位置清晰可见。足月时，层粘连蛋白存在于基质以及滋养层和血管基底膜中。血小板反应蛋白 I、生腱蛋白 C 和弹性蛋白的分布更为受限。据报道，纤维化与缺血有关，因此在三种不同的氧浓度下评估了培养的足月和妊娠早期胎盘成纤维细胞的 ECM 产生。纤连蛋白和 IV 型胶原蛋白的表达比 1 型胶原蛋白、原纤维蛋白 I 或血小板反应蛋白 1 更强，而层粘连蛋白和弹性蛋白的产量非常低。降低氧张力导致纤连蛋白和 IV 型胶原蛋白的产生选择性增加。因此，长期缺氧可能会导致 ECM 成分的定量和定性变化。 (C) 2003 Elsevier Science Ltd. 保留所有权利。
Database mining revealed 102 extracellular matrix (ECM) genes amongst about 10 000 mRNA species expressed in human placenta, and these were classified into collagens (23), non-collagenous glycoproteins (59) and proteoglycans (23). A panel of antibodies to selected collagens and glycoproteins was used to examine ECM distribution in the placental villous stroma. Collagens I and IV, fibronectin and fibrillin I were abundant in first trimester and term tissue. Some areas lacked collagen 1, while collagen IV was clearly evident in interstitial locations. At term, laminin was present in the stroma as well as in trophoblastic and vascular basement membranes. Thrombospondin I, tenascin C and elastin showed more restricted distributions. Fibrosis has been reported in association with ischaemia, so ECM production by cultured term and first trimester placental fibroblasts was evaluated at three different oxygen concentrations. Fibronectin and collagen IV were more strongly expressed than collagen 1, fibrillin I or thrombospondin 1, while the production of laminin and elastin was very low. Reducing the oxygen tension led to a selective increase in fibronectin and collagen IV production. Thus both quantitative and qualitative alterations in ECM composition may be expected to accompany prolonged hypoxia. (C) 2003 Elsevier Science Ltd. All rights reserved.