Effect of Fucoxanthin Alone and in Combination with D-glucosamine Hydrochloride on Carrageenan/kaolin-induced Experimental Arthritis in Rats
Effect of Fucoxanthin Alone and in Combination with D-glucosamine Hydrochloride on Carrageenan/kaolin-induced Experimental Arthritis in Rats
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DOI：
10.1002/ptr.5093
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发表时间：
2014-07-01
影响因子：
7.2
通讯作者：
Zhong, Laifu
中科院分区：
文献类型：
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作者：
Gong, Dezheng;Chu, Wanjiang;Zhong, Laifu
本研究旨在观察岩藻黄素（FUCO）单独及与盐酸氨基葡萄糖（GAH）联合应用对角叉菜胶/高岭土诱导的大鼠炎性关节炎模型的影响，并探讨其作用机制。检查关节肿胀、肌肉重量比（%）、组织病理学检查和评分以及蛋白多糖降解。分别采用酶联免疫吸附试验、免疫印迹分析和Griess试剂分析膝关节滑膜组织提取物中促炎性白细胞介素（IL-1 β）和肿瘤坏死因子（TNF-α）水平、环氧合酶-2（考克斯-2）和诱导型一氧化氮合酶（iNOS）蛋白表达以及一氧化氮（NO）水平。FUCO和FUCO+ GAH不仅可以显著降低膝关节肿胀程度并防止肌肉萎缩，还可以显著减轻滑膜组织中的炎症、软骨侵蚀和蛋白聚糖损失。FUCO+ GAH的效果优于GAH或FUCO。FUCO单独和FUCO+ GAH可显著抑制膝关节滑膜组织提取物中考克斯-2和iNOS蛋白表达的上调，降低IL-1 β和TNF-α水平，并减少NO产生。这些结果表明，FUCO是一种有效的抗关节炎剂，通过抗炎机制。FUCO可能通过抗炎作用增强GAH对大鼠关节炎的治疗作用。版权所有（C）2013约翰威利父子有限公司
The objective of the present study was to investigate the effect of the fucoxanthin (FUCO) alone and in combination with glucosamine hydrochloride (GAH) on carrageenan/kaolin-induced inflammatory arthritis model in rats and to explore its underlying mechanisms. Joint swelling, muscle weight ratio (%), histopathological examination and scoring, and proteoglycan degradation were examined. Pro-inflammatory interleukin (IL-1 beta) and tumor necrosis (TNF-alpha) levels, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase(iNOS) protein expression and nitric oxide (NO) level in knee synovial tissue extract were analyzed using enzyme-linked immunosorbent assay, western blotting analysis, and Griess reagent assay, respectively. FUCO and FUCO+ GAH not only may significantly reduce degrees of knee joint swelling and prevent against muscle atrophy, but also may significantly attenuate inflammation in synovial tissue, cartilage erosion, and proteoglycan loss. The efficacies of FUCO+ GAH were stronger than that of GAH or FUCO. FUCO alone and FUCO+ GAH can significantly inhibit upregulation of COX-2 and iNOS protein expressions, decrease of IL-1 beta and TNF-alpha levels, and reduce NO production in knee synovial tissue extract. These results indicated that FUCO is an effective anti-arthritis agent through an antiinflammation mechanism. FUCO may enhance therapeutic effect of GAH on rat arthritis through mechanism of antiinflammation. Copyright (C) 2013 John Wiley & Sons, Ltd.