Mucin glycoarray in gastric and gallbladder epithelia.
Mucin glycoarray in gastric and gallbladder epithelia.
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DOI：
10.1186/1477-3163-6-10
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发表时间：
2007-06-12
影响因子：
--
通讯作者：
Halagowder D
中科院分区：
文献类型：
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作者：
Ganesh IM;Subramani D;Halagowder D
粘蛋白是重要的细胞保护性糖蛋白，在不同的病理状态下，胃上皮粘蛋白的变化已被描述。本研究的目的是评估粘蛋白在更好地理解胃癌进展和胆囊结石形成中的假定有用性。福尔马林固定石蜡包埋的胃活检标本和手术切除的胆囊组织样本进行切片。进行阿尔新蓝（AB）染色以鉴定唾液粘蛋白（在pH 2.5下染成蓝色）和磺粘蛋白（在pH 1.0下染成棕色），然后进行过碘酸-希夫（PAS）染色以使中性粘蛋白可视化（染成品洋红）。在正常胃粘膜和胆囊粘膜中，中性粘蛋白占优势，而在肠上皮化生、胃癌和胆囊结石中，酸性粘蛋白明显增加，提示硫酸粘蛋白在胆囊结石形成中的作用大于中性粘蛋白，唾液酸粘蛋白和硫酸粘蛋白在肿瘤的进展和转移中起重要作用。我们的结果挑战糖生物学家更深入地阐明粘蛋白在胃恶性肿瘤和胆结石形成中的作用。
Mucins are critical cytoprotective glycoproteins and alterations of epithelial gastric mucins have been described in different pathological conditions. The purpose of the present study was to evaluate the putative usefulness of mucins in understanding the progression of gastric cancer and gallstone formation in a better perspective. Formalin-fixed paraffin-embedded gastric biopsy specimens and surgically resected gallbladder tissue samples were sectioned. Alcian Blue (AB) staining was performed to identify sialomucins (staining blue at pH 2.5) and sulfomucins (staining brown at pH 1.0) and then Periodic acid-Schiff's (PAS) staining to visualize the neutral mucins (staining magenta). In normal gastric and gallbladder mucosae, we found that neutral mucins were predominant, whereas in intestinal metaplasia, gastric carcinoma and stone-containing gallbladder, a significant increase of acidic mucins was found. We suggest that the sulfomucins have a greater role in gallstone formation than the neutral mucins and also that the sialomucins and sulfomucins play an important role in cancer progression and metastasis. Our results challenge the glycobiologists to delve deeper in elucidating the role of mucins in gastric malignancy and in gallstone formation.