Alzheimer's disease: glycolytic pretreatment dramatically enhances immunolabeling of senile plaques and cerebrovascular amyloid substance.
Alzheimer's disease: glycolytic pretreatment dramatically enhances immunolabeling of senile plaques and cerebrovascular amyloid substance.
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阿尔茨海默病：糖酵解预处理显着增强老年斑和脑血管淀粉样物质的免疫标记。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
M. Mazzuca
中科院分区：
文献类型：
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作者：
N. Behrouz;A. Defossez;A. Delacourte;P. Hublau;M. Mazzuca
在阿尔茨海默病中，有三种类型的病理病变被硫黄素染色：神经原纤维缠结、老年斑和淀粉样血管。我们使用了抗β蛋白淀粉样蛋白A4和抗tau蛋白抗血清，并比较了免疫标记和硫黄染色。抗tau蛋白仅检测到神经原纤维缠结；抗- pa4免疫染色老年斑和淀粉样血管。糖酵解预处理（2%定期酸过夜或糖苷酶消化）显著增强老年斑、淀粉样血管和以前未检测到的细胞外物质的抗- pa4免疫标记；神经原纤维缠结未进行免疫染色。因此，糖酵解预处理暴露了淀粉样蛋白中埋藏的表位，是一种很好的免疫染色扩增方法。糖与β -淀粉样蛋白A4之间相互作用的性质尚不清楚。
In Alzheimer's disease, three types of pathologic lesions are stained by thioflavin: neurofibrillary tangles, senile plaques, and amyloidaceous vessels. We have used anti-beta protein amyloid A4 and anti-tau protein antisera and compared immunolabeling with thioflavin staining. Anti-tau detected only neurofibrillary tangles; anti-beta-PA4 immunostained senile plaques and amyloidaceous vessels. Glycolytic pretreatment (2% periodic acid overnight or glycosidases digestion) dramatically enhanced the anti-beta-PA4 immunolabeling of senile plaques, amyloidaceous vessels, and a previously undetected extracellular substance; neurofibrillary tangles were never immunostained. Therefore, glycolytic pretreatment exposes buried epitopes in the amyloid and is a good method for amplification of immunostaining. The nature of the interaction between saccharides and beta-protein amyloid A4 is unknown.