Microporous Dermal-Like Electrospun Scaffolds Promote Accelerated Skin Regeneration
Microporous Dermal-Like Electrospun Scaffolds Promote Accelerated Skin Regeneration
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DOI：
10.1089/ten.tea.2013.0645
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发表时间：
2014-09-01
影响因子：
4.1
通讯作者：
Bellis, Susan L.
中科院分区：
文献类型：
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作者：
Bonvallet, Paul P.;Culpepper, Bonnie K.;Bellis, Susan L.
本研究的目的是合成皮肤替代品，将天然细胞外基质（ECM）分子与具有良好机械性能的合成聚合物混合。为此，支架由胶原蛋白I（col）和聚（ε-己内酯）（PCL）电纺，然后机械地引入孔以促进成纤维细胞浸润，随后用ECM填充孔。第七十章：30 col/PCL比率被确定为为真皮成纤维细胞生长提供最佳支持，并且确定了160 mm的孔径，其使得成纤维细胞能够用天然基质分子（包括纤连蛋白和胶原蛋白I）浸润和填充孔。机械测试70：具有160 mm孔的30 col/PCL支架显示出1.4 MPa的拉伸强度，并且支架还表现出低收缩率（
The goal of this study was to synthesize skin substitutes that blend native extracellular matrix (ECM) molecules with synthetic polymers which have favorable mechanical properties. To this end, scaffolds were electrospun from collagen I (col) and poly(e-caprolactone) (PCL), and then pores were introduced mechanically to promote fibroblast infiltration, and subsequent filling of the pores with ECM. A 70: 30 col/PCL ratio was determined to provide optimal support for dermal fibroblast growth, and a pore diameter, 160 mm, was identified that enabled fibroblasts to infiltrate and fill pores with native matrix molecules, including fibronectin and collagen I. Mechanical testing of 70: 30 col/PCL scaffolds with 160 mm pores revealed a tensile strength of 1.4 MPa, and the scaffolds also exhibited a low rate of contraction (